Pseudogenes Functions and Protocols /
Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality,...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Edition: | 1st ed. 2014. |
| Series: | Methods in Molecular Biology,
1167 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-0835-6 |
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Table of Contents:
- Computational Methods for Pseudogene Annotation Based on Sequence Homology.- Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content
- Computational Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation
- Whole Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS
- Methods to Study the Occurrence and the Evolution of Pseudogenes Through a Phylogenetic Approach
- Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing,0 RNA Amplification for Pseudogene Detection Using RNA-Seq.- GENCODE Pseudogenes
- Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features
- Proteomics Techniques for the Detection of Translated Pseudogenes
- Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation
- Pseudogenes: A Novel Source of Trans Acting Antisense RNAs
- Pseudogene-Derived Endogenous siRNAs and Their Function
- Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay
- Targeted and Robust Amplification of Mitochondrial DNA in the Presence of Nuclear Encoded Mitochondrial Pseudogenes Using Φ29 DNA Polymerases
- Discrimination of Pseudogene and Parental Gene DNA Methylation Using Allelic Bisulphite Sequencing Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long Range PCR on Genomic DNA
- PMS2 Gene Mutational Analysis: RNA-Based Direct cDNA Sequencing to Circumvent Pseudogene Interference
- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.