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Directed Evolution Library Creation Methods and Protocols /

Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational a...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Gillam, Elizabeth M.J. (Editor, http://id.loc.gov/vocabulary/relators/edt), Copp, Janine N. (Editor, http://id.loc.gov/vocabulary/relators/edt), Ackerley, David. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Springer, 2014.
Edition:2nd ed. 2014.
Series:Methods in Molecular Biology, 1179
Subjects:
Human genetics.
Evolutionary biology.
Genetic engineering.
Human Genetics.
Evolutionary Biology.
Genetic Engineering.
Online Access:https://doi.org/10.1007/978-1-4939-1053-3
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Table of Contents:
  • Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution
  • Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis
  • Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli
  • The Sequence Saturation Mutagenesis (SeSaM) Method
  • Generation of Effective Libraries by Neutral Drift
  • Site Saturation Mutagenesis
  • Iterative Saturation Mutagenesis (ISM): A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution
  • Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR)
  • OmniChange: Simultaneous Site-Saturation of up to Five Codons
  • Random Insertional–Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations
  • Transposon-Based Approaches for Generating Novel Molecular Diversity during Directed Evolution
  • Restriction Enzyme-Mediated DNA Family Shuffling
  • Assembly of Designed Oligonucleotides (ADO): A Useful Tool in Synthetic Biology for Creating High Quality Combinatorial DNA Libraries
  • One-Pot, Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR)
  • USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology
  • ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes
  • Generating Random Circular Permutation Libraries
  • Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate and Diversity
  • The Mutagenesis Assistant Program
  • Computational Tools for Designing Smart Libraries
  • Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies
  • Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP
  • Non-Contiguous SCHEMA Protein Recombination
  • Engineering Proteins by Reconstructing Evolutionary Adaptive Paths.

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