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Patch-Clamp Methods and Protocols

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Martina, Marzia. (Editor, http://id.loc.gov/vocabulary/relators/edt), Taverna, Stefano. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2014.
Edition:2nd ed. 2014.
Series:Methods in Molecular Biology, 1183
Subjects:
Neurosciences.
Human physiology.
Human Physiology.
Online Access:https://doi.org/10.1007/978-1-4939-1096-0
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Table of Contents:
  • History of Electrophysiology and the Patch-Clamp.- Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
  • Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers
  • State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
  • Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch® 96 Using Instant Frozen Cells
  • Planar Patch Clamp for Neuronal Networks–Considerations and Future Perspectives
  • Methods for the Study of Synaptic Receptor Functional Properties
  • Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity
  • Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
  • Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
  • Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices
  • Outside-Out ‘Sniffer-patch’ Clamp Technique for in situ Measures of Neurotransmitter Release
  • A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices
  • Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
  • Synaptic Connectivity in Engineered Neuronal Networks
  • Modeling of Action Potential Generation in NG108-15 Cells
  • Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
  • Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion
  • Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
  • Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents
  • Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
  • Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
  • Principles of Single-Channel Kinetic Analysis.

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