Affinity Chromatography Methods and Protocols /

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols,  mo...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Reichelt, Senta. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:3rd ed. 2015.
Series:Methods in Molecular Biology, 1286
Subjects:
Online Access:https://doi.org/10.1007/978-1-4939-2447-9
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Table of Contents:
  • Affinity Chromatography: A Historical
  • Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography
  • One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography
  • Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes
  • Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand
  • Cell Affinity Separations on Microfluidic Devices
  • Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins
  • The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins
  • Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins
  • Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins
  • Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products
  • Mixed-bed Affinity Chromatography: Principles And Methods
  • Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules
  • Introduction to Macroporous Cryogels
  • Cryogels with Affinity Ligands as Tools in Protein Purification
  • Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography
  • Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption
  • A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum
  •  Molecularly Imprinted Cryogels for Human Serum Albumin Depletion
  • Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties
  • Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin
  • Accurate protein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples
  • Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis
  • Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography
  • Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies.