Mycobacteria Protocols
Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Ausgabe: | 3rd ed. 2015. |
| Schriftenreihe: | Methods in Molecular Biology,
1285 |
| Schlagworte: | |
| Online Zugang: | https://doi.org/10.1007/978-1-4939-2450-9 |
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Inhaltsangabe:
- Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly
- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis
- RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria
- Fractionation and Analysis of Mycobacterial Proteins
- Lipid and Lipoarabinomannan Isolation and Characterization
- Metabolomics of Mycobacterium tuberculosis
- Electroporation of Mycobacteria
- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination
- Construction of Conditional Knockdown Mutants in Mycobacteria
- Mycobacterial Recombineering
- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria
- Genetic Dissection of Mycobacterial Biofilms
- Measuring Efflux and Permeability in Mycobacteria
- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy
- Antimicrobial Susceptibility Testing for Mycobacterium sp.
- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis
- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis
- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis
- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis
- Macrophage Infection Models for Mycobacterium tuberculosis
- Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis
- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids
- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum
- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays. .