Mycobacteria Protocols

Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.  Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids...

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Tác giả của công ty: SpringerLink (Online service)
Tác giả khác: Parish, Tanya. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt), Roberts, David M. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt)
Định dạng: Điện tử eBook
Ngôn ngữ:English
Được phát hành: New York, NY : Springer New York : Imprint: Humana, 2015.
Phiên bản:3rd ed. 2015.
Loạt:Methods in Molecular Biology, 1285
Những chủ đề:
Truy cập trực tuyến:https://doi.org/10.1007/978-1-4939-2450-9
Các nhãn: Thêm thẻ
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Mục lục:
  • Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly
  • Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis
  • RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria
  • Fractionation and Analysis of Mycobacterial Proteins
  • Lipid and Lipoarabinomannan Isolation and Characterization
  • Metabolomics of Mycobacterium tuberculosis
  • Electroporation of Mycobacteria
  • Targeted Gene Knockout and Essentiality Testing by Homologous Recombination
  • Construction of Conditional Knockdown Mutants in Mycobacteria
  • Mycobacterial Recombineering
  • In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria
  • Genetic Dissection of Mycobacterial Biofilms
  • Measuring Efflux and Permeability in Mycobacteria
  • Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy
  • Antimicrobial Susceptibility Testing for Mycobacterium sp.
  • Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis
  • Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis
  • A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis
  • Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis
  • Macrophage Infection Models for Mycobacterium tuberculosis
  • Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis
  • Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids
  • Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum
  • Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  .