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DNA Replication Methods and Protocols /

Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Vengrova, Sonya. (Editor, http://id.loc.gov/vocabulary/relators/edt), Dalgaard, Jacob. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:2nd ed. 2015.
Series:Methods in Molecular Biology, 1300
Subjects:
Medical genetics.
Gene Function.
Online Access:https://doi.org/10.1007/978-1-4939-2596-4
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Table of Contents:
  • A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells
  • Microscopy Techniques to Examine DNA Replication in Fission Yeast
  • High-Resolution Analysis of Mammalian DNA Replication Units
  • Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing
  • Measuring DNA Content by Flow Cytometry in Fission Yeast
  • Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast
  • Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast
  • Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection
  • Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo
  • Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae
  • Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin
  • Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors
  • Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism
  • Single-Molecule Observation Of Prokaryotic DNA Replication
  • Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast
  • Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
  • Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.

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