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C. elegans Methods and Applications /

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand....

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Biron, David. (Editor, http://id.loc.gov/vocabulary/relators/edt), Haspel, Gal. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:2nd ed. 2015.
Series:Methods in Molecular Biology, 1327
Subjects:
Human genetics.
Animal genetics.
Animal models in research.
Human Genetics.
Animal Genetics and Genomics.
Animal Models.
Online Access:https://doi.org/10.1007/978-1-4939-2842-2
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Table of Contents:
  • Library Construction for Mutation Identification by Whole-Genome Sequencing
  • Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes
  • Genetic Methods for Cellular Manipulations in C. elegans
  • A Fusion PCR Method for Expressing Genetic Tools in C. elegans
  • Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI)
  • Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System
  • Observing and Quantifying Fluorescent Reporters
  • Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior
  • Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans
  • High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans
  • Electron Tomography Methods for C. elegans
  • Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
  • Tracking Single C. elegans using a USB Microscope on a Motorized Stage
  • An Imaging System for C. elegans Behavior
  • A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae
  • Sampling and Isolation of C. elegans from the Natural Habitat
  • A Primer on Prototyping
  • A Primer on Quantitative Modeling.

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