DNA-Protein Interactions Principles and Protocols /
This fourth edition reflects the most recent technical advances in DNA-protein interaction research. With fully updated chapters that describe techniques proven by their continuous value, the volume also many new chapters have been added that mostly deal with larger-scale experiments, reflecting rec...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 4th ed. 2015. |
| Series: | Methods in Molecular Biology,
1334 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-2877-4 |
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Table of Contents:
- Electrophoretic Mobility Shift Assay Using Radiolabeled DNA Probes
- In Vitro DNase I Footprinting
- Determining the Architecture of a Protein/DNA Complex by Combining FeBABE Cleavage Analyses, 3-D Printed Structures, and the ICM Molsoft Program
- In Cellulo DNA Analysis: LMPCR Footprinting
- Southwestern Blotting Assay
- Single-Molecule Approaches for the Characterization of Riboswitch Folding Mechanisms
- Probing of Nascent Riboswitch Transcripts
- Functional Studies of DNA-Protein Interactions Using FRET Techniques
- Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5' Rapid Amplification of cDNA Ends (5'-RACE)
- Analysis of DNA Supercoiling Induced by DNA-Protein Interactions
- Precise Identification of DNA-Binding Proteins Genomic Location by Exonuclease Coupled Chromatin Immunoprecipitation (ChIP-exo)
- The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA
- Individual and Sequential Chromatin Immunoprecipitation Protocols
- Chromatin Endogenous Cleavage (ChEC) as a Method to Quantify Protein Interaction with Genomic DNA in Saccharomyces cerevisiae
- Selection and Validation of Spacer Sequences for CRISPR-Cas9 Genome Editing and Transcription Regulation in Bacteria
- Detection of Short-Range DNA Interactions in Mammalian Cells Using High-Resolution Circular Chromosome Conformation Capture Coupled to Deep Sequencing
- Global Mapping of Open Chromatin Regulatory Elements by Formaldehyde-Assisted Isolation of Regulatory Elements Followed by Sequencing (FAIRE-seq)
- Aggregate and Heatmap Representations of Genome-Wide Localization Data Using VAP, a Versatile Aggregate Profiler
- Circular Dichroism for the Analysis of Protein-DNA Interactions
- Quantitative Investigation of Protein-Nucleic Acid Interactions by Biosensor Surface Plasmon Resonance
- Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX.