Developmental Biology Protocols Volume III /
The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cu...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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| Edition: | 1st ed. 2000. |
| Series: | Methods in Molecular Biology,
137 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592590667 |
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| 245 | 1 | 0 | |a Developmental Biology Protocols |h [electronic resource] : |b Volume III / |c edited by Rocky S. Tuan, Cecilia W. Lo. |
| 250 | |a 1st ed. 2000. | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press : |b Imprint: Humana, |c 2000. | |
| 300 | |a XXVI, 522 p. |b online resource. | ||
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| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 137 | |
| 505 | 0 | |a Developmental Biology Protocols -- Manipulation Of Developmental Gene Expression and Function -- Ectopic Expression in Drosophila -- Clonal Analysis in the Examination of Gene Function in Drosophila -- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos -- Application of Functional Blocking Antibodies -- Analysis of Gene Expression -- Gene Expression Analyzed by Ribonuclease Protection Assay -- Relative Reverse Transcription-Polymerase Chain Reaction -- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control -- In Situ PCR Detection of HIV Expression in the Human Placenta -- Gene Expression Analysis by In Situ Hybridization -- Radio-Isotopic In Situ Hybridization on Tissue Sections -- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry -- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development -- Multicolor Whole-Mount In Situ Hybridization -- Methods for Double Detection of Gene Expression -- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins -- Monoclonal Antibodies in the Analysis of Embryonic Development -- Models of Morphogenesis and Development -- Mesoderm Induction in Xenopus -- Amphibian Organizer Activity -- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery -- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration -- The Chimeric Human/Mouse Model of Angiogenesis -- Analysis of Embryonic Vascular Morphogenesis -- Epithelial-Mesenchymal Interactions -- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning -- Palate Development -- In Vitro Models and Analysis of Differentiation and Development -- In Vitro Fertilization -- Trophoblast Differentiation -- Bone Marrow-Derived Mesenchymal Progenitor Cells -- Identification, Characterization, and Differentiation of Human Prostate Cells -- Preparation of Chick Striated Muscle Cultures -- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm -- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation -- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells -- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis -- Skeletogenesis -- Studying Early Hematopoiesis Using Avian Blastoderm Cultures -- Isolation and Culture of Mouse Germ Cells -- Cadherin-Mediated Cell-Cell Interactions -- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins -- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells -- Developmental Models Of Diseases -- Pax3 and Vertebrate Development -- Genetic-Engineered Models of Skeletal Diseases I -- Genetic-Engineered Models of Skeletal Diseases II -- Transgenic Mouse Models of Craniofacial Disorders. | |
| 520 | |a The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis. | ||
| 650 | 0 | |a Developmental biology. | |
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| 700 | 1 | |a Tuan, Rocky S. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
| 700 | 1 | |a Lo, Cecilia W. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
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| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 137 | |
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