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Colorectal Cancer Methods and Protocols /

Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying...

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Ente Autore: SpringerLink (Online service)
Altri autori: Powell, Steven M. (Redattore, http://id.loc.gov/vocabulary/relators/edt)
Natura: Elettronico eBook
Lingua:English
Pubblicazione: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edizione:1st ed. 2001.
Serie:Methods in Molecular Medicine, 50
Soggetti:
Oncology  .
Oncology.
Accesso online:https://doi.org/10.1385/1592590845
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Sommario:
  • Microdissection of Histologic Sections
  • Isolation of a Purified Epithelial Cell Population from Human Colon
  • Xenografting Human Colon Cancers
  • Comparative Genomic Hybridization Technique
  • Fluorescence In Situ Hybridization
  • Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer
  • The SURF Technique
  • Microsatellite Instability Testing
  • Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer
  • Mutation Detection in Colorectal Cancers
  • Mutation Detection inTGF-? Receptors
  • Direct Analysis for Familial Adenomatous Polyposis Mutations
  • I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer
  • Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
  • Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
  • Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
  • 17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations
  • 18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
  • Genetic Analysis Using Microarrays
  • Cclooxygenase-2 (COX-2) Protein Expression by Western Blotting
  • The Immunohistochemical Method
  • Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids
  • Adhesion Signaling Through Integrins
  • Electrophoretic Mobility Shift Assay (EMSA)
  • Transfection Assays for Transformation of Model Colonic Cell Lines
  • Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay
  • Gene Transfer into the Colonic Mucosa.

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