Epithelial Cell Culture Protocols
Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures...
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| Other Authors: | |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
188 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/159259185X |
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| 001 | 978-1-59259-185-5 | ||
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| 245 | 1 | 0 | |a Epithelial Cell Culture Protocols |h [electronic resource] / |c edited by Clare Wise. |
| 250 | |a 1st ed. 2002. | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press : |b Imprint: Humana, |c 2002. | |
| 300 | |a XIV, 406 p. |b online resource. | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 188 | |
| 505 | 0 | |a Human Lens Epithelial Cell Culture -- Human Airway Epithelial Cell Culture -- Rat Gastric Mucosal Epithelial Cell Culture -- Thymic Epithelial Cell Culture -- Bile Duct Epithelial Cell Culture -- Liver Epithelial Cell Culture -- Pulmonary Epithelial Cell Culture -- Prostate Epithelial Cell Isolation and Culture -- A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier -- The Blood-CSF Barrier in Culture -- An In Vitro Model of Differentiated Human Airway Epithelia -- Primary Mouse Keratinocyte Culture -- Analyzing Apoptosis in Cultured Epithelial Cells -- Keratins as Markers of Epithelial Cells -- Epithelial Cell Integrins -- Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium -- Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces -- Transplantation of Cultured Epithelial Cells -- An Outgrowth Culture System of Normal Human Epithelium -- Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract -- Applications of Epithelial Cell Culture in Studies of Drug Transport -- X-Ray Microanalysis of Epithelial Cells in Culture -- ENU Mutagenesis of Rat Mammary Epithelial Cells -- ENU-Induced Ovarian Cancer -- Measurement of Membrane Capacitance in Epithelial Monolayers -- Assessing Epithelial Cell Confluence by Spectroscopy -- Co-Cultivation of Liver Epithelial Cells with Hepatocytes -- Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium -- Establishing Epithelial-Immune Cell Co-Cultures -- Explant Cultures of Embryonic Epithelium -- Bacterial Interactions with Host Epithelium In Vitro. | |
| 520 | |a Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. | ||
| 650 | 0 | |a Respiratory organs—Diseases. | |
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| 700 | 1 | |a Wise, Clare. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
| 710 | 2 | |a SpringerLink (Online service) | |
| 773 | 0 | |t Springer Nature eBook | |
| 776 | 0 | 8 | |i Printed edition: |z 9781617372414 |
| 776 | 0 | 8 | |i Printed edition: |z 9781489938824 |
| 776 | 0 | 8 | |i Printed edition: |z 9780896038936 |
| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 188 | |
| 856 | 4 | 0 | |u https://doi.org/10.1385/159259185X |
| 912 | |a ZDB-2-PRO | ||
| 950 | |a Springer Protocols (Springer-12345) | ||