Antibody Phage Display Methods and Protocols /
Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensi...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
178 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592406 |
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Table of Contents:
- Overview of Antibody Phage-Display Technology and Its Applications
- Standard Protocols for the Construction of Fab Libraries
- Standard Protocols for the Construction of scFv Libraries
- Broadening the Impact of Antibody Phage Display Technology
- Construction of Large Naïve Fab Libraries
- Construction of Polyclonal Antibody Libraries Using Phage Display
- Antigen-Driven Stimulation of B-Lymphocytes In Vitro
- 8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion
- Panning of Antibody Phage-Display Libraries
- Selection of Antibodies Against Biotinylated Antigens
- Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
- Blocking Immunodominant Epitopes by Competitive Deselection
- Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy
- Screening of Phage-Expressed Antibody Libraries by Capture Lift
- Antibody-Guided Selection Using Capture-Sandwich ELISA
- Proximity-Guided (ProxiMol) Antibody Selection
- Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
- Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques
- Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs
- Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments
- Selection of Antibodies Based on Antibody Kinetic Binding Properties
- Selection of Functional Antibodies on the Basis of Valency
- Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis
- Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement
- Error-Prone Polymerase Chain Reaction for Modification of scFvs
- Use of Escherichia coli Mutator Cells to Mature Antibodies
- Chain Shuffling to Modify Properties of Recombinant Immunoglobulins
- Generation of Bispecific and Tandem Diabodies
- High-Level Periplasmic Expression and Purification of scFvs
- Periplasmic Expression and Purification of Recombinant Fabs
- Expression of Antibody Fragments in Pichia pastoris
- Expression of VHH Antibody Fragments in Saccharomyces cerevisiae
- Intrabodies
- 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
- 35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells.