RT-PCR Protocols
RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical resear...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
193 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/159259283X |
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Table of Contents:
- RT-PCR in Biomedicine
- The Basics of RT-PCR
- Highly Sensitive Detection and Analysis of mRNA
- Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples
- Detection of mRNA Expression and Alternative Splicing in a Single Cell
- Nested RT-PCR
- Quantitative RT-PCR
- Quantitative RT-PCR
- Rapid Development of a Quantitative-Competitive (qc) RT-PCR Assay Using a Composite Primer Approach
- Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products
- Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR
- Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes
- Detection and Analysis of RNA Viruses
- Detection and Quantification of the Hepatitis C Viral Genome
- Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCR
- RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus
- In Situ Localization of mRNA Expression
- in situ Immuno-PCR
- RT-PCR from Laser-Capture Microdissected Samples
- Mycobacterium paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by LCM in Cases of Crohn’s Disease
- RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization
- Differential mRNA Expression
- Amplified RNA for Gene Array Hybridizations
- Semi-Quantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR
- Genetic Analysis
- Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay
- RT-PCR in Immunology
- Detection of Clonally Expanded T-Cells by RT-PCR-SSCP and Nucleotide Sequencing of T-Cell Receptor ?-CDR3 Regions
- Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B-Cells
- Generation of Murine scFv Intrabodies from B-Cell Hybridomas
- Quantitation of mRNA Levels by RT-PCR in Cells Purified by FACS
- RT-PCR in Anti-Sense Technology
- Detection of Anti-Sense RNA Transcripts by Anti-Sense RT-PCR
- RT-PCR in cDNA Cloning
- RT-PCR in cDNA Library Construction
- An RT-PCR-Based Protocol for the Rapid Generation of Large, Representative cDNA Libraries for Expression Screening.