Animal Cell Biotechnology Methods and Protocols /

In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book cover...

Mô tả đầy đủ

Đã lưu trong:
Chi tiết về thư mục
Tác giả chính: Jenkins, Nigel. (Tác giả, http://id.loc.gov/vocabulary/relators/aut)
Tác giả của công ty: SpringerLink (Online service)
Định dạng: Điện tử eBook
Ngôn ngữ:English
Được phát hành: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Phiên bản:1st ed. 1999.
Loạt:Methods in Biotechnology, 8
Những chủ đề:
Truy cập trực tuyến:https://doi.org/10.1385/0896035476
Các nhãn: Thêm thẻ
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
Mục lục:
  • Enabling Technologies
  • Setting Up a New Cell Culture Laboratory
  • Adaptation of Mammalian Cells to Growth in Serum-Free Media
  • Viral Evaluation of Animal Cell Lines Used in Biotechnology
  • Molecular Methods
  • Optimizing Gene Expression in Mammalian Cells
  • Cytogenetic Characterization of Recombinant Cells
  • Immortalization Strategies for Mammalian Cells
  • Mouse Monoclonal Antibodies
  • Cell Bank Preparation and Characterization
  • DNA Fingerprinting and Characterization of Animal Cells
  • Cell Evaluation Protocols
  • Cell Counting and Viability Measurements
  • Monitoring Animal Cell Growth and Productivity by Flow Cytometry
  • Measurement of Cell Death in Culture
  • Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism
  • Specialist Techniques
  • Culturing Animal Cells in Fluidized Bed Reactors
  • GPI-Anchored Fusion Proteins
  • Harvesting GPI-Anchored Proteins From CHO Cells
  • Hematopoietic Cells for Cellular and Gene Therapy
  • Hematopoietic Cells for Cellular and Gene Therapy
  • Cytotoxicity Testing Using Cell Lines
  • Product Evaluation Protocols
  • Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells
  • Control of Proteolysis in Cell Culture
  • Monitoring Recombinant Glycoprotein Heterogeneity
  • Measurement of Cell-Culture Glycosidase Activity.