Immunocytochemical Methods and Protocols
The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth ods has spread to a wide array of biological disciplines. Early applica tions focused on the detec...
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| Diğer Yazarlar: | |
| Materyal Türü: | Elektronik Ekitap |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1995.
|
| Edisyon: | 1st ed. 1995. |
| Seri Bilgileri: | Methods in Molecular Biology,
34 |
| Konular: | |
| Online Erişim: | https://doi.org/10.1385/089603285X |
| Etiketler: |
Etiketle
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| LEADER | 05081nam a22004335i 4500 | ||
|---|---|---|---|
| 001 | 978-1-59259-521-1 | ||
| 003 | DE-He213 | ||
| 005 | 20200709061735.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s1995 xxu| s |||| 0|eng d | ||
| 020 | |a 9781592595211 |9 978-1-59259-521-1 | ||
| 024 | 7 | |a 10.1385/089603285X |2 doi | |
| 050 | 4 | |a QR180-189.5 | |
| 072 | 7 | |a MJCM |2 bicssc | |
| 072 | 7 | |a MED044000 |2 bisacsh | |
| 072 | 7 | |a MJCM |2 thema | |
| 082 | 0 | 4 | |a 616.079 |2 23 |
| 245 | 1 | 0 | |a Immunocytochemical Methods and Protocols |h [electronic resource] / |c edited by Lorette C. Javois. |
| 250 | |a 1st ed. 1995. | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press : |b Imprint: Humana, |c 1995. | |
| 300 | |a XII, 435 p. |b online resource. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 34 | |
| 505 | 0 | |a Overview of Antibody Use in Immunocytochemistry -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration -- Purification of Antibodies Using Ion-Exchange Chromatography -- Purification of Antibodies Using Affinity Chromatography -- Purification of Antibodies Using Protein A-Sepharose and FPLC -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Overview of Cell Fixation and Permeabilization -- Preparation of Frozen Sections for Analysis -- Processing of Cell-Touch Preparations -- Processing of Cytological Specimens -- Processing of Tissue-Culture Cells -- Processing of Tissue Specimens -- Overview of Fluorophores -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method -- The Avidin-Biotin Complex(ABC) Method -- Labeled Avidin Binding (LAB) Method* -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- ELISA on Attachment-Dependent or Suspension Grown Cells -- Use of Immunogold with Silver Enhancement -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Assay for Phagocytosis -- Assay for Filamentous Actin -- Assay for Chemoattractant Binding -- Assay for Oxidative Metabolism -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-Embedding Labeling Methods -- Postembedding Labeling Methods -- Overview of Fluorescence Photomicrography -- Overview of Fluorescence Analysis with the Confocal Microscope -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Nucleic Acid Immunocytochemistry -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes -- Overview of Automated Immunostainers -- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors. | |
| 520 | |a The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth ods has spread to a wide array of biological disciplines. Early applica tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disci plines, volumes have been published focusing on the specific applica tion of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Meth ods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry. | ||
| 650 | 0 | |a Immunology. | |
| 650 | 1 | 4 | |a Immunology. |0 https://scigraph.springernature.com/ontologies/product-market-codes/B14000 |
| 700 | 1 | |a Javois, Lorette C. |e editor. |4 edt |4 http://id.loc.gov/vocabulary/relators/edt | |
| 710 | 2 | |a SpringerLink (Online service) | |
| 773 | 0 | |t Springer Nature eBook | |
| 776 | 0 | 8 | |i Printed edition: |z 9780896032859 |
| 776 | 0 | 8 | |i Printed edition: |z 9781489940063 |
| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 34 | |
| 856 | 4 | 0 | |u https://doi.org/10.1385/089603285X |
| 912 | |a ZDB-2-PRO | ||
| 950 | |a Springer Protocols (Springer-12345) | ||