Baculovirus Expression Protocols

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Bibliographic Details
Main Author: Richardson, Christopher D. (Author, http://id.loc.gov/vocabulary/relators/aut)
Corporate Author: SpringerLink (Online service)
Format: Electronic eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1995.
Edition:1st ed. 1995.
Series:Methods in Molecular Biology, 39
Subjects:
Online Access:https://doi.org/10.1385/0896032728
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Table of Contents:
  • to the Molecular Biology of Baculoviruses
  • Baculovirus Transfer Vectors Miguel LÓpez- Ferber
  • Insect Cell- Culture Techniques in Serum- Containing Medium
  • Insect Cell Culture in Serum- Free Media
  • Transfection Techniques for Producing Recombinant Baculoviruses
  • Selection of Recombinant Baculoviruses by Visual Screening
  • Production of recombinant baculoviruses using linearized viral DNA
  • Rapid procedures for the isolation and PCR analysis of recombinant baculovirus
  • Production of recombinant baculoviruses using rapid screening vectors that contain the gene for ß -Galactosidase
  • Fast isolation of recombinant baculovirus by antibody screening
  • Continuous foreign gene expression in transformed lepidopteran insect cells
  • Scale-Up of recombinant virus and protein production in Stirred-Tank reactors
  • Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor
  • Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors
  • Expression of Foreign Proteins in Trichoplusia ni Larvae
  • Use of Baculoviruses as Biological Insecticides
  • Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors
  • Expression of the Hemagglutinin Protein of Influenza Virus
  • Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose
  • Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-L Reactor
  • Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG)
  • Comparison of Different Cell Lines for the Production of Recombinant Baculovirus Proteins
  • Development of Lepidopteran Cell Lines.