YAC Protocols
Yeast artificial chromosomes (YACs) have their origins in the molecular genetic analysis of the yeast Saccharomyces cerevisiae. The construction of self-maintaining genetic elements from isolated frag ments of the yeast genome defined DNA sequences necessary for chro mosome function has provided t...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1996.
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| Edition: | 1st ed. 1996. |
| Series: | Methods in Molecular Biology,
54 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896033139 |
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Table of Contents:
- Generation of Large Insert YAC Libraries
- YAC Library Storage and Transport
- YAC Library Screening I
- YAC Library Screening II
- Cloning of Human Telomeres in Saccharomyces cerevisiae
- Purification of YAC-Containing Total Yeast DNA
- Restriction Analysis of YACs
- RARE-Cleavage Analysis of YACs
- YAC Localization by Fluorescence In Situ Hybridization
- Alu-PCR Fingerprinting of YACs
- Detection of Chimerism in YAC Clones
- Amplification with Arbitrary Primers
- End Rescue from YACs Using the Vectorette
- Isolation of YAC Ends by Plasmid Rescue
- End-Rescue of YAC Clone Inserts by Inverse PCR
- Covering YAC-Cloned DNA with Phages and Cosmids
- Fragmentation and Integrative Modification of YACs
- Targeting Mutations to YACs by Homologous Recombination
- Reconstruction of Large Genomic Segments of DNA by Meiotic Recombination Between YACs
- Genomic Reconstruction by Mitotic Recombination of YACs
- Amplification of the Copy Number of YACs
- Transfer of YAC Clones to New Yeast Hosts
- Use of ACEDB as a Database for YAC Library Data Management
- YAC Transfer into Mammalian Cells by Cell Fusion
- YAC Transfer by Microinjection
- Transfection of Mammalian Cells via Lipofection
- The Isolation of cDNAs by Hybridization of YACs to cDNA Libraries
- cDNA Selection with YACs
- Markers, Selection, and Media in YAC Cloning.