Plant Cell Electroporation And Electrofusion Protocols
Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile ge...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1995.
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| Edition: | 1st ed. 1995. |
| Series: | Methods in Molecular Biology,
55 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896033287 |
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Table of Contents:
- Theory and Instrumentation
- Electroporation Theory
- Effects of Pulse Length and Strength on Electroporation Efficiency
- Instrumentation
- Electroporation Protocols
- Electroporation of Agrobacterium tumefaciens
- Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii
- Pollen Electrotransformation in Tobacco
- Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA
- Electroporation of Brassica
- Transformation of Maize by Electroporation of Embryos
- Transient Gene Expression Analysis in Electroporated Maize Protoplasts
- Reporter Genes and Transient Assays for Plants
- Electrofusion Protocols
- Electrofusion of Plant Protoplasts
- Protoplast Electrofusion and Regeneration in Potato
- Polymer-Supported Electrofusion of Protoplasts.