Parasite Genomics Protocols
Parasitic disease remains an important health issue in most regions of the world and constitutes a major cause of morbidity and mortality in developing countries. In Parasite Genomics Protocols, pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analy...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edition: | 1st ed. 2004. |
| Series: | Methods in Molecular Biology,
270 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592597939 |
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Table of Contents:
- Sequencing Strategies for Parasite Genomes
- Annotation of Parasite Genomes
- Parasite Genome Databases and Web-Based Resources
- Expressed Sequence Tags
- Expressed Sequence Tags
- Positive Selection Scanning of Parasite DNA Sequences
- RACE and RAGE Cloning in Parasitic Microbial Eukaryotes
- Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis
- Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates
- Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics
- Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays
- DNA Content Analysis on Microarrays
- Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection
- Transfection of the Human Malaria Parasite Plasmodium falciparum
- A PCR-Based Method for Gene Deletion and Protein Tagging in Trypanosoma brucei
- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference
- In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons
- Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments
- Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose
- Chromosome Fragmentation in Leishmania
- FISH Mapping for Helminth Genome
- Fiber-FISH: Fluorescence In Situ Hybridization on Stretched DNA
- Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions
- From Genomes to Vaccines for Leishmaniasis.