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Hematopoietic Stem Cell Protocols

With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Ce...

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书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Bunting, Kevin D. (Editor, http://id.loc.gov/vocabulary/relators/edt)
格式: 电子 电子书
语言:English
出版: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
版:2nd ed. 2008.
丛编:Methods in Molecular Biology, 430
主题:
Biochemistry.
Cell biology.
Hematology.
Molecular biology.
Biochemistry, general.
Cell Biology.
Molecular Medicine.
在线阅读:https://doi.org/10.1007/978-1-59745-182-6
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书本目录:
  • Overview
  • The Expanding Tool Kit for Hematopoietic Stem Cell Research
  • Stem Cell Enrichment and Analysis
  • Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties
  • Mobilization of Hematopoietic Stem and Progenitor Cells in Mice
  • Transmigration of Human CD34+ Cells
  • Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells
  • Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry
  • In Vitro Assays and Differentiation
  • In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells
  • Hematopoietic Development of Human Embryonic Stem Cells in Culture
  • In Vitro Human T Cell Development Directed by Notch–Ligand Interactions
  • In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C
  • Transplantation Assays for Mouse and Human HSC
  • Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection
  • Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects
  • Isolation and Functional Characterization of Side Population Stem Cells
  • Transplantation of Chimeric Fetal Liver to Study Hematopoiesis
  • Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair
  • Genetic Modification of HSCs and Imaging Engraftment
  • Retroviral Transduction of Murine Hematopoietic Stem Cells
  • Lentiviral Gene Transduction of Mouse and Human Stem Cells
  • Retroviral Integration Site Analysis in Hematopoietic Stem Cells
  • A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells
  • Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.

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