Genomics Protocols

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characteri...

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Dades bibliogràfiques
Autor corporatiu: SpringerLink (Online service)
Altres autors: Starkey, Mike. (Editor, http://id.loc.gov/vocabulary/relators/edt), Elaswarapu, Ramnath. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electrònic eBook
Idioma:English
Publicat: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edició:2nd ed. 2008.
Periòdiques:Methods in Molecular Biology, 439
Matèries:
Accés en línia:https://doi.org/10.1007/978-1-59745-188-8
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Taula de continguts:
  • Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield
  • Scanning for DNA Variants by Denaturant Capillary Electrophoresis
  • Identification of SNPs, or Mutations in Sequence Chromatograms
  • BeadArray-Based Genotyping
  • Microsatellite-Based Candidate Gene Linkage Analysis Studies
  • Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH)
  • Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification
  • Application of Microarrays for DNA Methylation Profiling
  • Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray
  • Transcriptional Profiling of Small Samples in the Central Nervous System
  • Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays
  • Expression Profiling of microRNAs in Cancer Cells: Technical Considerations
  • Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry
  • The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease
  • Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis
  • Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags
  • Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics
  • Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line
  • Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression
  • Coimmunoprecipitation and Proteomic Analyses
  • Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes
  • Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
  • Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation
  • Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells
  • GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data
  • Comparative Genomics-Based Prediction of Protein Function
  • Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing.