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PCR Protocols

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and trick...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Park, Daniel J. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:3rd ed. 2011.
Series:Methods in Molecular Biology, 687
Subjects:
Human genetics.
Nucleic acids.
Laboratory medicine.
Human Genetics.
Nucleic Acid Chemistry.
Laboratory Medicine.
Online Access:https://doi.org/10.1007/978-1-60761-944-4
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Table of Contents:
  • Setup of a PCR Laboratory
  • Long Range PCR with a DNA Polymerase Fusion
  • Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures
  • Lariat-Dependent Nested PCR for Flanking Sequence Determination
  • CODEHOP PCR and CODEHOP PCR Primer Design
  • Sequencing of Difficult DNA Regions by SAM Sequencing
  • A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis
  • Quantitation of MicroRNAs by Real-Time RT-qPCR
  • High-Throughput SuperSAGE
  • Deep Cap Analysis of Gene Expression (Deep CAGE)
  • Linking Emulsion PCR Haplotype Analysis
  • PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure
  • The Many Faces of MLPA
  • Assessing Gene-Specific Methylation Using HRM-Based Analysis
  • Alu PCR
  • Asynchronous PCR
  • Novel Applications of PCR through the Use of DNA Substrates
  • Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support
  • Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR
  • Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis
  • Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries
  • GLOBE: Analysis of DNA-Protein Interaction Analysis
  • PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
  • Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution.

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