Integrin and Cell Adhesion Molecules Methods and Protocols /
Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cance...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
757 |
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| Online Access: | https://doi.org/10.1007/978-1-61779-166-6 |
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Table of Contents:
- Overview: Assays for Studying Integrin-Dependent Cell Adhesion
- Cell Adhesion Assays
- Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes
- Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes
- Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands
- Cell-Free Ligand-Binding Assays for Integrin LFA-1
- Overview: Structural Biology of Integrins
- Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography
- Electron Microscopic Imaging of Integrin
- An NMR Method to Study Protein-Protein Interactions
- Single Molecule Methods to Study Cell Adhesion Molecules
- Overview: Imaging in the Study of Integrins
- Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals
- Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer
- High-resolution Fluorescence Microscopy to Study Transendothelial Migration
- Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes
- Overview of Integrin Signaling in the Immune System
- Rap1 and Integrin Inside-out Signaling
- Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis
- Talin and Signaling Through Integrins
- Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins
- Overview: Studying Integrins In Vivo
- A Method for the Generation of Conditional Gene-Targeted Mice
- T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations
- In Vivo Quantitative Proteomics: the SILAC-Mouse
- Analysis of Chemotaxis in Dictyostelium
- Integrins in Tumor Angiogenesis & Lymphangiogenesis
- PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody
- Integrin-targeted Nanoparticles for siRNA Delivery.- Humanized Mice for Studying Human Leukocyte Integrins In Vivo.