Epigenetics Protocols
The field of epigenetics has played a major role at the forefront not only of molecular biology, but also of medical genetics and clinical medicine. Few disciplines have experienced growth comparable to that which we have witnessed for epigenetics in the past decade. The goal of Epigenetics Protoco...
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| Formato: | Recurso Eletrônico livro eletrônico |
| Idioma: | English |
| Publicado em: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edição: | 2nd ed. 2011. |
| coleção: | Methods in Molecular Biology,
791 |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-61779-316-5 |
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Sumário:
- Advances in Epigenetic Technology
- DNA Methylation Detection: Bisulfite Genomic Sequencing Analysis
- Methylation-specific PCR (MSP)
- Analyzing DNA Methylation Using Bisulfite Pyrosequencing
- Closed-tube PCR Methods for Locus Specific DNA Methylation Analysis
- A Combined Bisulfite Restriction Analysis (COBRA) Bioinformatics Tool: Methyl-typing
- SIRPH: an HPLC-based SNuPE for Quantitative Methylation Measurement at Specific CpG Sites
- Restriction Landmark Genome Scanning (RLGS)
- Methylated DNA Immunoprecipitation (MeDIP) Genome-wide Analysis
- Methylated-CpG Island Recovery Assay (MIRA)
- Global DNA Methylation Analysis Using the Luminometric Methylation Assay, LUMA
- Inhibition of DNA Methylation in Somatic Cells
- DNA Methyltransferase Assays
- A Chromatin Immunoprecipitation Protocol for Small Cell Numbers
- Native Chromatin Immunoprecipitation (nChIP)
- Q-PCR in Combination with ChIP Assays to Detect Changes in Chromatin Acetylation
- Sequential Chromatin Immunoprecipitation (SeqChIP) Assay and Analysis
- Combined Chromatin Immunoprecipitation and Bisulfite Methylation Sequencing (ChIP-BMS) Analysis
- Studying RNA-protein Interactions in vivo by RNA Immunoprecipitation (RIP)
- Using ChIP-seq Technology to Generate High-resolution Profiles of Histone Modifications
- Mapping Open Chromatin with Formaldehyde-assisted Isolation of Regulatory Elements (FAIRE)
- Inhibition of Histone Deacetylases
- Computational Methods for Epigenetic Analysis - the Protocol of Computational Analysis for Modified Methylation-specific Digital Karyotyping (MMSDK) Based on Massively Parallel Sequencing.