Astrocytes Methods and Protocols /
Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS. Astrocytes: Methods and P...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
814 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-61779-452-0 |
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Table of Contents:
- Astrocytes: Multi-talented Stars of the Central Nervous System.-Neurogenic Astrocytes and their Glycoconjugates: not just “Glue” Anymore
- Heterogeneity of Astrocytic Form and Function.-Preparation of Mixed Glial Cultures from Postnatal Rat Brain.-Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia.-Derivation of Microglia-free Astrocyte Cultures from Neural Stem Cells.-Isolation and Culture of Spinal Cord Astrocytes.-Isolation and Culture of Astrocytes from the Retina and Optic Nerve.-Primary Cultures of Astrocytes from Fetal Bovine Brain.-Isolation and Cultivation of Porcine Astrocyte
- Isolation and Culture of Human Astrocytes.-Cell Culturing of C. elegans Glial Cells for the Assessment of Cytosolic Ca2+ Dynamics.-Astrocyte Polarization and Wound Healing in Culture:Studying Cell Adhesion Molecules.-An in Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity.-Use of Gel Zymography to Examine Matrix Metalloproteinase (gelatinase) Expression in Brain Tissue or in Primary Glial Cultures.-ELISA Methodology to Quantify Astrocyte Production of Cytokines/Chemokines In Vitro.-Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures.-Examining Potassium Channel Function in Astrocytes.-Connexin Based Channels in Astrocytes: How to Study their Properties.-Isotope-based Quantitation of Uptake, Release and Metabolism of Glutamate and Glucose in Cultured Astrocytes.-An in Vitro Model of Reactive Astrogliosis and its Effect on Neuronal Growth.-A Neuron-Astrocyte Co-Culture System to Investigate Astrocyte Secreted Factors in Mouse Neuronal Development.-Co-cultures of Neurons and Astrocytes as a Model for Examining Hypoxia-induced Neuronal Death.-Use of Astrocyte-Microglial Co-cultures to Examine the Regulatory Influence of Astrocytes on Microglial Activation.-Astrocyte-Schwann Cell Co-Culture Systems.-An in Vitro Assay to Examine Oligodendrocyte Precursor Cell Migration on Astrocytes.-Investigation of Astrocyte-Oligodendrocyte Interactions in Human Cultures.-An Improved in vitro Blood-Brain Barrier Model: Rat Brain Endothelial Cells Co-Cultured with Astrocytes.-In Vitro Models of the Blood-Brain Barrier.-An in Vitro Model of Ischemic Stroke.-Pericyte Co-Culture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions.-Reprogramming of Postnatal Astroglia of the Mouse Neocortex into Functional Synapse-Forming Neurons.-Assessment of Glial Function in the in Vivo Retina.-Imaging in Vivo Astrocyte/Endothelial Cell Interactions at the Blood-Brain Barrier.-Transgenic Techniques for Cell Ablation or Molecular Deletion to Investigate Functions of Astrocytes and Other GFAP-Expressing Cell Types.-Use of Cre-Lox technology to analyze integrin functions in astrocytes.-In Vivo Imaging of Ca2+ Signaling in Astrocytes Using Two Photon Laser Scanning Fluorescent Microscopy. .