Ubiquitin Family Modifiers and the Proteasome Reviews and Protocols /
The ubiquitin-proteasome system (UPS) and ubiquitin-related modifiers are not only involved in cellular protein quality control but also in the regulation of many fundamental cellular processes/pathways as well as in their disease-relevant aberrations. Ubiquitin Family Modifiers and Proteasome: Revi...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
832 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-61779-474-2 |
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Table of Contents:
- Three Decades of Studies to Understand the Functions of the Ubiquitin Family
- Structure and Evolution of Ubiquitin and Ubiquitin-Related Domains
- Deciphering Tissue-Specific Ubiquitylation by Mass Spectrometry
- Analysis of Cellular SUMO and SUMO-Ubiquitin Hybrid Conjugates
- Recombinant Reconstitution of Sumoylation Reactions In vitro
- Reconstitution of SUMO-Dependent Ubiquitylation In vitro
- Detection and Analysis of FAT10 Modification
- Isolation of NEDDylated Proteins in Human Cells
- The ISG15 Conjugation System
- Pupylation: Proteasomal Targeting by a Protein Modifier in Bacteria
- Role of UbF Family Modifiers and their Binding Proteins in Cell Signaling
- Isolation of Ubiquitylated Proteins using Tandem Ubiquitin-Binding Entities (TUBEs)
- Using Linkage-Specific Monoclonal Antibodies to Analyze Cellular Ubiquitination
- Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity
- Synthesis and Analysis of K11-Linked Ubiquitin Chains
- Synthesis and Analysis of Linear Ubiquitin Chains
- Detection and Quantitation of SUMO Chains by Mass Spectrometry
- Bioinformatical Detection of Recognition Factors for Ubiquitin and SUMO
- Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners
- Identifying and Studying Ubiquitin Receptors by NMR
- Exploring the Role of p97 and its UBX-Domain Cofactors Through Identification of Their Interacting Proteins
- Assembly and Function of the Proteasome
- Using Native Gel Electrophoresis and Phosphofluoroimaging to Analyse GFP-tagged Proteasomes
- Disulfide Engineering to Map Subunit Interactions in the Proteasome and Other Macromolecular Complexes
- Using DNA Damage Sensitivity Phenotypes to Characterize Mutations Affecting Proteasome Function
- Analysing Properties of Proteasome Inhibitors using Kkinetic and X-Ray Crystallographic Studies
- Immunoproteasome-Specific Inhibitors and Their Application
- Binding of Ubiquitin Conjugates to Proteasomes as Visualized with Native Gels
- Affinity Purification of Mmammalian 26S Proteasomes Using an Ubiquitin-Like Domain
- Using siRNA Techniques to Dissect Proteasome Assembly Pathways in Mammalian Cells
- Reconstitution of PA700, the 19S Regulatory Particle, from Purified Precursor Complexes
- Cellular Responses to Misfolded Proteins and Protein Aggregates
- Live-Cell Imaging of Ubiquitin-Proteasome System Function
- Analysis of Chaperone-assisted Ubiquitylation
- Use of CPY* and its Derivatives to Study Protein Quality Control in Various Cell Compartments
- Assays to Measure ER-Associated Degradation in Yeast
- SDS-PAGE Techniques to Study Ubiquitin-Like Conjugation Systems in Yeast Autophagy
- Analysis of Ubiquitin-Dependent Proteolysis in C. elegans
- Structural Insights into Functional Modes of Proteins Involved in Ubiquitin Family Pathways
- Identification and Application of NEDD8 E1 Inhibitors
- Formation of Ubiquitin Dimers via Azide Alkyne Click Reaction
- Synthesis of Atypical Diubiquitin Chains
- TIPI - TEV Protease-Mediated Induction of Protein Instability
- PROTAC-Induced Proteolytic Targeting.