Difference Gel Electrophoresis (DIGE) Methods and Protocols /
Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavil...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
854 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-61779-573-2 |
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Table of Contents:
- DIGE: Past and Future
- The Basics of 2D DIGE
- Multifluorescence 2D Gel Imaging and Image Analysis
- Assessing Signal-to-Noise in Quantitative Proteomics: Multivariate Statistical Analysis in DIGE Experiments
- Analysis of Proteins Using DIGE and MALDI Mass Spectrometry
- Synthesis and Validation of Cyanin-Based Dyes for DIGE
- 2D DIGE Saturation Labeling for Minute Sample Amounts
- Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE
- Analysis of Protein Post-Translational Modifications Using DIGE-Based Proteomics
- Comparative Analyses of Protein Complexes by Blue Native DIGE
- 2D DIGE Analysis of Protein Extracts from Muscle Tissue
- Combination of Highly Efficient Hexapeptide Ligand Library-Based Sample Preparation with 2D DIGE for the Analysis of the Hidden Human Serum/Plasma Proteome
- 2D DIGE Analysis of Serum after Fractionation by ProteoMinerTM Beads
- Study Design in DIGE-Based Biomarker Discovery
- Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery
- Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma
- 2D DIGE for the Analysis of RAMOS Cells Sub-Proteomes
- Application of Saturation Labeling in Lung Cancer Proteomics
- Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE
- Method for Protein Subfractionation of Cardiovascular Tissues before DIGE Analysis
- Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus (T2DM) Mouse Models
- Evaluating the Efficacy of Subcellular Fractionation of Blast Cells Using Live Cell Labeling and 2D DIGE
- DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method
- Native DIGE of Fluorescent Plant Protein Complexes
- An Overview of 2D DIGE Analysis of Marine (Environmental) Bacteria
- Application of 2D DIGE in Animal Proteomics.