Antibody Methods and Protocols
The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using i...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
901 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-61779-931-0 |
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Table of Contents:
- Antigen Presentation for the Generation of Binding Molecules
- Recombinant Antibodies and In Vitro Selection Technologies
- Phage Display
- Generation of Human Fab Libraries for Phage Display
- Selection of Human Fab Libraries by Phage Display
- Ribosome Display
- Hybridoma Technology for the Generation of Monoclonal Antibodies
- The Application of Transgenic Mice for Therapeutic Antibody Discovery
- Production of Human or Humanized Antibodies in Mice
- Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
- Kinetic Screening in the Antibody Development Process
- Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection
- Determination of Antibody Glycosylation by Mass Spectrometry
- Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format
- PEGylation of Antibody Fragments for Half-Life Extension
- Bispecific Antibody Derivatives Based on Full-Length IgG Formats
- Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells
- Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function
- Class-Specific Effector Functions of Therapeutic Antibodies.