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siRNA Design Methods and Protocols /

The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi.  siRNA Design: Methods and Protocols offers...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Taxman, Debra J. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edition:1st ed. 2013.
Series:Methods in Molecular Biology, 942
Subjects:
Biotechnology.
Human genetics.
Human Genetics.
Online Access:https://doi.org/10.1007/978-1-62703-119-6
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Table of Contents:
  • What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
  • Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
  • Designing Functional siRNA with Reduced Off-Target Effects
  • Design and Screening of siRNAs Against Highly Structured RNA Targets
  • Engineering Small Interfering RNAs by Strategic Chemical Modification
  • The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
  • The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
  • Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
  • Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
  • Strategies for Designing and Validating Immunostimulatory siRNAs
  • Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
  • Short Hairpin RNA-Mediated Gene Silencing
  • Design of Lentivirally Expressed siRNAs
  • Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
  • Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
  • Production and Application of Long dsRNA in Mammalian Cells
  • Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
  • Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
  • Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
  • Downregulation of Plant Genes with MiRNA Induced Gene Silencing.

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