Basic Cell Culture Protocols
At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Four...
Saved in:
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
|
| Edition: | 4th ed. 2013. |
| Series: | Methods in Molecular Biology,
946 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-62703-128-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- 1. Detection of Mycoplasma Contaminations
- 2. Eradication of Mycoplasma Contaminations
- 3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination
- 4. Classical and Molecular Cytogenetic Analysis
- 5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle
- 6. The Development of T Lymphocytes in Fetal Thymus Organ Culture
- 7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
- 8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
- 9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media
- 10. Isolation and Characterization of Mouse Side Population Cells
- 11. Stem Cell Identification by DyeCycle Violet Side Population Analysis
- 12. Isolation and Characterization of Cancer Stem Cells In Vitro
- 13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets
- 14. Generation and Characterization of Murine Alternatively Activated Macrophages
- 15. Human Long Term Culture Initiating Cell (LTC-IC) Assay
- 16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells
- 17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
- 18. Studying Leukocyte Recruitment Under Flow Conditions
- 19. Generation and Establishment of Murine Adherent Cell Lines
- 20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture
- 21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone
- 22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture
- 23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells
- 24. Human Prostate Epithelial Cell Cultures
- 25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue
- 26. Isolation and Characterization of Human Hair Follicle Epithelial Cells
- 27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
- 28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
- 29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells
- 30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System
- 31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
- 32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates
- 33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells.