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Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols /

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into t...

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Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Rabinovich, Peter M. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronic eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edition:1st ed. 2013.
Series:Methods in Molecular Biology, 969
Subjects:
Human genetics.
Human Genetics.
Online Access:https://doi.org/10.1007/978-1-62703-260-5
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Table of Contents:
  • Cell Engineering with Synthetic Messenger RNA
  • In vitro Transcription of Long RNA Containing Modified Nucleosides
  • HPLC Purification of in vitro Transcribed Long RNA
  • Synthetic mRNAs with Superior Translation and Stability Properties
  • Non-Viral, Cationic Lipid-Mediated Delivery of mRNA
  • Light-Induced mRNA Transfection
  • Engineering B Cells with mRNA
  • Programming Human Dendritic Cells with mRNA
  • Large Volume Flow Electroporation of mRNA: Clinical Scale Process
  • Retrovirus-Based mRNA Transfer for Transient Cell Manipulation
  • mRNA Delivery to Human Dendritic Cells by Recombinant
  • Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy
  • Natural Killer Cell Reprogramming with Chimeric Immune Receptors
  • Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
  • Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies
  • Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA
  • Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
  • mRNA electroporation as a Tool for Immunomonitoring
  • mRNA PCR-Based Epitope Chase Method.

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