Antibody Engineering Volume 1
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. Antibody Engineering p...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2010.
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| Edition: | 2nd ed. 2010. |
| Series: | Springer Protocols Handbooks,
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| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-3-642-01144-3 |
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Table of Contents:
- Cloning of the Antigen-binding Site from Hybridoma
- Cloning of Variable Domains from Mouse Hybridoma by PCR
- Coning Hybridoma cDNA by RACE
- Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly
- Generation of Antibody Repertoires
- Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens
- Human Antibody Gene Libraries
- Synthetic Antibody Libraries
- Immune Libraries from Nonhuman Primates (NHP)
- Generation of Rabbit Immune Libraries
- Selection of Antibody Fragments from Combinatorial Libraries
- Immunotube Selections
- Phage Display and Selection in Microtitre Plates
- Phage Display and Selections on Biotinylated Antigens
- Phage Display and Subtractive Selection on Cells
- Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells
- Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA
- Yeast Display and Selections
- The Generation of Transgenic Mice Expressing Human Antibody Repertoires
- Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay
- Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries
- Engineering and Production of Immunoglobulins
- Aspects of Isotype Selection
- Generation of Heavy and Light Chains (Chimeric Antibodies)
- Humanising Antibodies by CDR Grafting
- Humanization by Resurfacing
- Human Antibodies by Guided Selection
- In Silico De-Immunization
- Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis
- Affinity Maturation by Random Mutagenesis and Phage Display
- Engineering of the Fc Region for Improved PK (FcRn Interaction)
- Antibody-Dependent Enzyme Prodrug Therapy (ADEPT)
- Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
- Expression of IgA Molecules in Mammalian Cells
- Antibody Characterization
- Expression of Complete Antibodies in Transgenic Plants
- Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast
- Expression of IgG Antibodies in Mammalian Cells
- Protein A/G Chromatography
- Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology
- Epitope Mapping by Printed Peptide Libraries
- Antibody Epitope Mapping Using Yeast Display
- Size Exclusion Chromatography
- Structural Characterization of Antibodies by Mass Spectrometry
- Antibody Glycans Characterization
- Affinity Measurements by Competition ELISA
- Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis
- Affinity Measurements Using Quartz Crystal Microbalance (QCM)
- Affinity Measurements with Radiolabeled Antibodies
- Neutralization Tests
- Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo
- Competitive ELISA
- Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
- Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay
- Binding Studies with Flow Cytometry.