Effects of alginate hydrocolloid film permeated with vicenin 2 on wound healing in streptozotocin-induced diabetics Sprague Dawley rats
Wound healing impairment is one of the debilitating complications caused by diabetes mellitus (DM) which leads to morbidity and mortality. Previous preliminary report of our team has showed that Vicenin-2 (VCN-2) bioactive compound which possesses potent wound healing properties is identified fro...
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Online Access: | http://ethesis.upm.edu.my/id/eprint/13881/1/IB%202018%2040%20T.pdf |
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| Summary: | Wound healing impairment is one of the debilitating complications caused by
diabetes mellitus (DM) which leads to morbidity and mortality. Previous preliminary
report of our team has showed that Vicenin-2 (VCN-2) bioactive compound which
possesses potent wound healing properties is identified from Moringa oleifera
extract (MO). The general objective of this study is to determine the wound healing
efficacy of VCN-2 hydrocolloid film on diabetic rats. Firstly, the cytotoxicity and
medicinal effects of VCN-2 was investigated through in vitro studies. A new sodium
alginate (SA) film formulation contained VCN-2 was then optimized and
developed, the various physicochemical tests were conducted on the developed
VCN-2 film. Subsequently, the VCN-2 film was then tested in streptozotocin (STZ)
induced male Sprague Dawley rats. The diabetes induced rat models were
subjected to wound infliction and topical treatment of SA hydrocolloid film with
VCN-2 in respective doses (12.5, 25 and 50 μM) for 14 days. Finally, the animals
were sacrificed for wound healing assessment. Results indicated that VCN-2
hydrocolloid film has significantly reduced the production of pro-inflammatory
cytokines and mediators (IL-6, IL-1β, TNF-α, NF-κB, iNOS and COX-2), while
enhancing the production of healing growth factors (VEGF and TGF-β) in wound
samples. During diabetic wound healing, the VCN-2 hydrocolloid film had
influence inflammation phase by suppressing pro-inflammatory cytokines and
proliferation phase by inducing growth factor expression. It has ameliorated
prolong inflammatory process and enhanced healing through VEGF and TGF-β
pathways. Taken together, VCN-2 hydrocolloid film is a promising new treatment
to enhance impaired wound healing in hyperglycemic condition which prompts
further investigation. |
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