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Role of Interleukin-33 during malaria infection in mouse model

Malaria remains as the most prevalent parasitic disease, exerting significant health and economic burden globally. This disease is associated with multitude of inflammatory mediators which often elicits severe immunopathological reactions. To circumvent this scenario, the role of Interleukin-33 (...

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Main Author: Abd Rachman Isnadi, Mohammad Faruq
Format: Thesis
Language:English
Published: 2018
Online Access:http://ethesis.upm.edu.my/id/eprint/14178/1/FPSK%28m%29%202018%2052%20T.pdf
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spelling oai:ethesis.upm.edu.my:14178 http://ethesis.upm.edu.my/id/eprint/14178/ Role of Interleukin-33 during malaria infection in mouse model Abd Rachman Isnadi, Mohammad Faruq Malaria remains as the most prevalent parasitic disease, exerting significant health and economic burden globally. This disease is associated with multitude of inflammatory mediators which often elicits severe immunopathological reactions. To circumvent this scenario, the role of Interleukin-33 (IL-33), a diverse, IL-1 related cytokine during malaria infection was investigated. Plasmodium berghei ANKA (PbA) malaria infection was initiated with 2 x 107 of parasitised red blood cells (PRBC) and established in the male Imprinting Control Region (ICR) mice as the model for murine malaria. Parasitaemia was measured throughout the course of infection. Enzyme- Linked Immunosorbent Assay (ELISA) was performed to determine the systemic, extracellular IL-33 level in the plasma, while Immunohistochemistry (IHC) was performed to assess the local, intracellular IL-33 expression in the major malariainfected organs comprised of the brain, heart, liver, lung, spleen, and kidney at: onset (day 1), middle (day 3), and critical phase (day 5) of malaria infection. Modulation and the outcomes of augmenting, neutralising, and antagonising of IL-33 in malaria were observed in the treated mice and harvested major malaria-infected organs through physical signs of illness and histopathological evaluation. It was observed that IL-33 was positively correlated with PRBC development at the onset phase of the systemic level, followed by upregulation at the critical phase of malaria infection in both systemic and local expression of the brain, lung, and spleen. Modulating IL-33 treatment reduced the PRBC sequestration and haemozoin deposition accompanied with the absence of haemorrhagic features in the brain, heart, and lung while prolonging the survival of the malaria model. Therefore, it is postulate that IL-33 renders significant roles as a pro-inflammatory cytokine in murine malaria model as an alarming cytokine and mechanical barriers signal. It is suggestive that more studies pertaining to IL-33 should be conducted in other diseases as immunotherapeutic target approaches. 2018-05 Thesis NonPeerReviewed text en http://ethesis.upm.edu.my/id/eprint/14178/1/FPSK%28m%29%202018%2052%20T.pdf Abd Rachman Isnadi, Mohammad Faruq (2018) Role of Interleukin-33 during malaria infection in mouse model. Masters thesis, Universiti Putra Malaysia. (FPSK(m) 2018 52).
institution UPM eTHESES
collection UPM eTHESES
language English
description Malaria remains as the most prevalent parasitic disease, exerting significant health and economic burden globally. This disease is associated with multitude of inflammatory mediators which often elicits severe immunopathological reactions. To circumvent this scenario, the role of Interleukin-33 (IL-33), a diverse, IL-1 related cytokine during malaria infection was investigated. Plasmodium berghei ANKA (PbA) malaria infection was initiated with 2 x 107 of parasitised red blood cells (PRBC) and established in the male Imprinting Control Region (ICR) mice as the model for murine malaria. Parasitaemia was measured throughout the course of infection. Enzyme- Linked Immunosorbent Assay (ELISA) was performed to determine the systemic, extracellular IL-33 level in the plasma, while Immunohistochemistry (IHC) was performed to assess the local, intracellular IL-33 expression in the major malariainfected organs comprised of the brain, heart, liver, lung, spleen, and kidney at: onset (day 1), middle (day 3), and critical phase (day 5) of malaria infection. Modulation and the outcomes of augmenting, neutralising, and antagonising of IL-33 in malaria were observed in the treated mice and harvested major malaria-infected organs through physical signs of illness and histopathological evaluation. It was observed that IL-33 was positively correlated with PRBC development at the onset phase of the systemic level, followed by upregulation at the critical phase of malaria infection in both systemic and local expression of the brain, lung, and spleen. Modulating IL-33 treatment reduced the PRBC sequestration and haemozoin deposition accompanied with the absence of haemorrhagic features in the brain, heart, and lung while prolonging the survival of the malaria model. Therefore, it is postulate that IL-33 renders significant roles as a pro-inflammatory cytokine in murine malaria model as an alarming cytokine and mechanical barriers signal. It is suggestive that more studies pertaining to IL-33 should be conducted in other diseases as immunotherapeutic target approaches.
format Thesis
author Abd Rachman Isnadi, Mohammad Faruq
spellingShingle Abd Rachman Isnadi, Mohammad Faruq
Role of Interleukin-33 during malaria infection in mouse model
author_facet Abd Rachman Isnadi, Mohammad Faruq
author_sort Abd Rachman Isnadi, Mohammad Faruq
title Role of Interleukin-33 during malaria infection in mouse model
title_short Role of Interleukin-33 during malaria infection in mouse model
title_full Role of Interleukin-33 during malaria infection in mouse model
title_fullStr Role of Interleukin-33 during malaria infection in mouse model
title_full_unstemmed Role of Interleukin-33 during malaria infection in mouse model
title_sort role of interleukin-33 during malaria infection in mouse model
publishDate 2018
url http://ethesis.upm.edu.my/id/eprint/14178/1/FPSK%28m%29%202018%2052%20T.pdf
_version_ 1819311527686045696
score 13.4562235

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