صورة الغلاف

Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production

Oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) are lignocellulosic biomass that abundantly generated in palm oil mills. However, the presence of these oil palm wastes has created a major disposal problem. Current treatment is either by mulching at the plantation of dumping at...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Ahmad Rizal, Nur Fatin Athirah
التنسيق: أطروحة
اللغة:English
منشور في: 2018
الوصول للمادة أونلاين:http://ethesis.upm.edu.my/id/eprint/14624/1/FBSB%202018%2065%20-%20T.pdf
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record_format eprints
spelling oai:ethesis.upm.edu.my:14624 http://ethesis.upm.edu.my/id/eprint/14624/ Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production Ahmad Rizal, Nur Fatin Athirah Oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) are lignocellulosic biomass that abundantly generated in palm oil mills. However, the presence of these oil palm wastes has created a major disposal problem. Current treatment is either by mulching at the plantation of dumping at side of the mill. Since these materials are rich in carbohydrate the OPEFB and OPMF have been widely reported as suitable raw materials to produce fermentable sugars. However, the presence of lignin and hemicellulose in their composition hinders the access of cellulase to hydrolyze cellulose. Effective pretreatments are required to reduce the recalcitrance of lignocellulosic structures and improve the fermentable sugars production. Combination of physico-chemical and biological pretreatment was proposed to enhance glucose production from OPEFB and OPMF. Physico-chemical pretreatment using superheated steam (SHS) was employed in this study as it can modify the lignocellulosic materials. Results showed SHS pretreatment alone had increased the percentage of cellulose by 13.4% for OPEFB and 19.4% for OPMF, and reduced hemicelluloses percentage to 18.7% and 21.3%, respectively. However, this SHS pretreatment could only generated 18.4% of glucose yield for OPEFB and 15.6% for OPMF. In order to enhance the glucose yield, combination pretreatments of SHS with laccase has been studied. Study showed that the best laccase loading for OPEFB was 100 U/g-substrate while for OPMF was 400 U/g-substrate. This raw size SHS + laccase pretreatment had enhanced 34.6% and 36.1% of glucose yield for OPEFB and OPMF, respectively. The delignification of OPEFB and OPMF was further improved by reducing the particle size to 2 mm, 1 mm, 0.5 mm and 0.25mm using a hammer mill after the SHS pretreatment and before treating with laccase. The reduction of size to 0.25 mm had improved the glucose yield by 71.5% for OPEFB and 63.0% for OPMF which is equivalent to 4.6-fold and 4.8- fold increment, respectively as compared to untreated substrates. To conclude, this study revealed that glucose yield was successfully enhanced by combining SHS with laccase pretreatment together with the size reduction of OPEFB and OPMF. 2018-07 Thesis NonPeerReviewed text en http://ethesis.upm.edu.my/id/eprint/14624/1/FBSB%202018%2065%20-%20T.pdf Ahmad Rizal, Nur Fatin Athirah (2018) Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production. Masters thesis, Universiti Putra Malaysia. (FBSB 2018 65).
institution UPM eTHESES
collection UPM eTHESES
language English
description Oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) are lignocellulosic biomass that abundantly generated in palm oil mills. However, the presence of these oil palm wastes has created a major disposal problem. Current treatment is either by mulching at the plantation of dumping at side of the mill. Since these materials are rich in carbohydrate the OPEFB and OPMF have been widely reported as suitable raw materials to produce fermentable sugars. However, the presence of lignin and hemicellulose in their composition hinders the access of cellulase to hydrolyze cellulose. Effective pretreatments are required to reduce the recalcitrance of lignocellulosic structures and improve the fermentable sugars production. Combination of physico-chemical and biological pretreatment was proposed to enhance glucose production from OPEFB and OPMF. Physico-chemical pretreatment using superheated steam (SHS) was employed in this study as it can modify the lignocellulosic materials. Results showed SHS pretreatment alone had increased the percentage of cellulose by 13.4% for OPEFB and 19.4% for OPMF, and reduced hemicelluloses percentage to 18.7% and 21.3%, respectively. However, this SHS pretreatment could only generated 18.4% of glucose yield for OPEFB and 15.6% for OPMF. In order to enhance the glucose yield, combination pretreatments of SHS with laccase has been studied. Study showed that the best laccase loading for OPEFB was 100 U/g-substrate while for OPMF was 400 U/g-substrate. This raw size SHS + laccase pretreatment had enhanced 34.6% and 36.1% of glucose yield for OPEFB and OPMF, respectively. The delignification of OPEFB and OPMF was further improved by reducing the particle size to 2 mm, 1 mm, 0.5 mm and 0.25mm using a hammer mill after the SHS pretreatment and before treating with laccase. The reduction of size to 0.25 mm had improved the glucose yield by 71.5% for OPEFB and 63.0% for OPMF which is equivalent to 4.6-fold and 4.8- fold increment, respectively as compared to untreated substrates. To conclude, this study revealed that glucose yield was successfully enhanced by combining SHS with laccase pretreatment together with the size reduction of OPEFB and OPMF.
format Thesis
author Ahmad Rizal, Nur Fatin Athirah
spellingShingle Ahmad Rizal, Nur Fatin Athirah
Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
author_facet Ahmad Rizal, Nur Fatin Athirah
author_sort Ahmad Rizal, Nur Fatin Athirah
title Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
title_short Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
title_full Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
title_fullStr Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
title_full_unstemmed Integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
title_sort integration of physico-chemical and enzymatic pretreatments of oil palm biomass for enhancement of glucose production
publishDate 2018
url http://ethesis.upm.edu.my/id/eprint/14624/1/FBSB%202018%2065%20-%20T.pdf
_version_ 1819311575425613824
score 13.4562235

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