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The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction

Nitric oxide (NO) is involved in many physiological responses in plants including defense mechanism against biotic and abiotic stresses. NO is known to be an important signaling molecule in protein S-nitrosylation which involves modification of cysteine thiols to generate S-nitrosothiol (SNO). Prote...

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Main Author: Mohd Nasir, Nurul Najihah
Format: Project Report
Language:English
Published: 2015
Online Access:http://ethesis.upm.edu.my/id/eprint/15480/1/FBSB%202015%20166%20-%20T.pdf
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spelling oai:ethesis.upm.edu.my:15480 http://ethesis.upm.edu.my/id/eprint/15480/ The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction Mohd Nasir, Nurul Najihah Nitric oxide (NO) is involved in many physiological responses in plants including defense mechanism against biotic and abiotic stresses. NO is known to be an important signaling molecule in protein S-nitrosylation which involves modification of cysteine thiols to generate S-nitrosothiol (SNO). Protein extraction from Fusarium oxysporum f. sp. cubense (Foc)-banana root tissue was performed by using a modified TCA-acetone extraction method. Detection of S-nitrosylated proteins in the protein extract following biotin switch assay results in detection of a single band approximately at 52 kDa. Berangan banana seedlings which was treated with NO donor, nitrosogluthathione (GSNO) and NO scavenger, (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) cPTIO prior to inoculation with Foc indicated that disease progression in GSNO treated Berangan banana seedlings was delayed. On the other hand, disease progression was shown to be accelerated in cPTIO-treated Berangan banana seedlings. This study shows that NO plays important role in basal resistance against Foc. 2015-06 Project Report NonPeerReviewed text en http://ethesis.upm.edu.my/id/eprint/15480/1/FBSB%202015%20166%20-%20T.pdf Mohd Nasir, Nurul Najihah (2015) The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction. Bachelor project report, Universiti Putra Malaysia. (FBSB 2015 166). (Submitted)
institution UPM eTHESES
collection UPM eTHESES
language English
description Nitric oxide (NO) is involved in many physiological responses in plants including defense mechanism against biotic and abiotic stresses. NO is known to be an important signaling molecule in protein S-nitrosylation which involves modification of cysteine thiols to generate S-nitrosothiol (SNO). Protein extraction from Fusarium oxysporum f. sp. cubense (Foc)-banana root tissue was performed by using a modified TCA-acetone extraction method. Detection of S-nitrosylated proteins in the protein extract following biotin switch assay results in detection of a single band approximately at 52 kDa. Berangan banana seedlings which was treated with NO donor, nitrosogluthathione (GSNO) and NO scavenger, (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) cPTIO prior to inoculation with Foc indicated that disease progression in GSNO treated Berangan banana seedlings was delayed. On the other hand, disease progression was shown to be accelerated in cPTIO-treated Berangan banana seedlings. This study shows that NO plays important role in basal resistance against Foc.
format Project Report
author Mohd Nasir, Nurul Najihah
spellingShingle Mohd Nasir, Nurul Najihah
The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
author_facet Mohd Nasir, Nurul Najihah
author_sort Mohd Nasir, Nurul Najihah
title The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
title_short The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
title_full The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
title_fullStr The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
title_full_unstemmed The role of nitric oxide in fusarium oxysporum f.sp. Cubense – banana interaction
title_sort role of nitric oxide in fusarium oxysporum f.sp. cubense – banana interaction
publishDate 2015
url http://ethesis.upm.edu.my/id/eprint/15480/1/FBSB%202015%20166%20-%20T.pdf
_version_ 1782703139201220608
score 12.935284

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