Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products

This study was conducted to screen for the antimicrobial activity of isolated LAB from dairy product and to identify the genera of the LAB. Four isolates of unknown bacteria were obtained from Mycology Laboratory at Biotech 2 with the permission from the supervisor. All the unknown bacteria were iso...

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Yazar: Ramli, Siti Nur Alia
Materyal Türü: Project Report
Dil:English
Baskı/Yayın Bilgisi: 2015
Online Erişim:http://ethesis.upm.edu.my/id/eprint/15488/1/FBSB%202015%20129%20-%20T.pdf
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spelling oai:ethesis.upm.edu.my:15488 http://ethesis.upm.edu.my/id/eprint/15488/ Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products Ramli, Siti Nur Alia This study was conducted to screen for the antimicrobial activity of isolated LAB from dairy product and to identify the genera of the LAB. Four isolates of unknown bacteria were obtained from Mycology Laboratory at Biotech 2 with the permission from the supervisor. All the unknown bacteria were isolated from 4 diferrent resources of dairy product. Among four isolates, only one showed the clear zone in the screening of antimicrobial activity against Escherichia coli and Listeria monocytogenes. The isolate was labelled as sample 1, it showed rod shape under the microscope and was revealed as the gram positive bacteria due its appearace in purple colour on gram staining test. Presumption test was done on sample 1 with a few series of biochemical test and it was identified as Lactobacillus sp. The LAB was tested using critical dilution method to determine the quantitatively amount of the antimicrobial activity on E. coli and L. monocytogenes. Regarding to this finding, it can be further commercialized on fighting the food disease associated by food borne pathogen and and may involve in the food preservative method. 2015 Project Report NonPeerReviewed text en http://ethesis.upm.edu.my/id/eprint/15488/1/FBSB%202015%20129%20-%20T.pdf Ramli, Siti Nur Alia (2015) Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products. Bachelor project report, Universiti Putra Malaysia. (FBSB 2015 129). (Submitted)
institution UPM eTHESES
collection UPM eTHESES
language English
description This study was conducted to screen for the antimicrobial activity of isolated LAB from dairy product and to identify the genera of the LAB. Four isolates of unknown bacteria were obtained from Mycology Laboratory at Biotech 2 with the permission from the supervisor. All the unknown bacteria were isolated from 4 diferrent resources of dairy product. Among four isolates, only one showed the clear zone in the screening of antimicrobial activity against Escherichia coli and Listeria monocytogenes. The isolate was labelled as sample 1, it showed rod shape under the microscope and was revealed as the gram positive bacteria due its appearace in purple colour on gram staining test. Presumption test was done on sample 1 with a few series of biochemical test and it was identified as Lactobacillus sp. The LAB was tested using critical dilution method to determine the quantitatively amount of the antimicrobial activity on E. coli and L. monocytogenes. Regarding to this finding, it can be further commercialized on fighting the food disease associated by food borne pathogen and and may involve in the food preservative method.
format Project Report
author Ramli, Siti Nur Alia
spellingShingle Ramli, Siti Nur Alia
Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
author_facet Ramli, Siti Nur Alia
author_sort Ramli, Siti Nur Alia
title Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
title_short Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
title_full Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
title_fullStr Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
title_full_unstemmed Identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
title_sort identification and screening of antimicrobial activity of lactic acid bacteria isolated from dairy products
publishDate 2015
url http://ethesis.upm.edu.my/id/eprint/15488/1/FBSB%202015%20129%20-%20T.pdf
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