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In Vitro Studies on the Virulence of Spodoptera litura Baculovirus

Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infectivi...

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Main Author: Mohammed Hussein, Syakira
Format: Thesis
Language:English
Published: 2003
Online Access:http://ethesis.upm.edu.my/id/eprint/2648/1/FSAS_2003_58_F.pdf
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spelling oai:ethesis.upm.edu.my:2648 http://ethesis.upm.edu.my/id/eprint/2648/ In Vitro Studies on the Virulence of Spodoptera litura Baculovirus Mohammed Hussein, Syakira Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infectivity of two genera of baculoviruses, Nucleopolyhedrosis virus (NPV) and Granulovirus (GV) to cultured lepidopteran cells. Production of baculoviruses depends on the ability of these cells to replicate in an optimal condition. Spodoptera litura baculovirus isolated locally was investigated for its virulence to two cell lines by inoculating with both forms of the virus, occlusion-body derived virions (PDV) and budded virus (BV), from SpltNPV and SpltGV. Efforts to develop cell cultures from the local Spodoptera litura for use in replicating these local baculoviruses was, however, unsuccessful due to cell deterioration or microbial contamination. A cell line from Spodoptera Jrugiperda (S£9) was susceptible to budded virus obtained directly from the insect, but not to viral progeny. While these results suggest Sf9 cells have potential for replicating these baculoviruses, the Spodoptera litura cell line, TUAT -Spli-221 appeared not to be susceptible. 2003-12 Thesis NonPeerReviewed application/pdf en http://ethesis.upm.edu.my/id/eprint/2648/1/FSAS_2003_58_F.pdf Mohammed Hussein, Syakira (2003) In Vitro Studies on the Virulence of Spodoptera litura Baculovirus. Masters thesis, Universiti Putra Malaysia. (FSAS 2003 58).
institution UPM eTHESES
collection UPM eTHESES
language English
description Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infectivity of two genera of baculoviruses, Nucleopolyhedrosis virus (NPV) and Granulovirus (GV) to cultured lepidopteran cells. Production of baculoviruses depends on the ability of these cells to replicate in an optimal condition. Spodoptera litura baculovirus isolated locally was investigated for its virulence to two cell lines by inoculating with both forms of the virus, occlusion-body derived virions (PDV) and budded virus (BV), from SpltNPV and SpltGV. Efforts to develop cell cultures from the local Spodoptera litura for use in replicating these local baculoviruses was, however, unsuccessful due to cell deterioration or microbial contamination. A cell line from Spodoptera Jrugiperda (S£9) was susceptible to budded virus obtained directly from the insect, but not to viral progeny. While these results suggest Sf9 cells have potential for replicating these baculoviruses, the Spodoptera litura cell line, TUAT -Spli-221 appeared not to be susceptible.
format Thesis
author Mohammed Hussein, Syakira
spellingShingle Mohammed Hussein, Syakira
In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
author_facet Mohammed Hussein, Syakira
author_sort Mohammed Hussein, Syakira
title In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
title_short In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
title_full In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
title_fullStr In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
title_full_unstemmed In Vitro Studies on the Virulence of Spodoptera litura Baculovirus
title_sort in vitro studies on the virulence of spodoptera litura baculovirus
publishDate 2003
url http://ethesis.upm.edu.my/id/eprint/2648/1/FSAS_2003_58_F.pdf
_version_ 1819310281768042496
score 13.4562235

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