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Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae

Although it has been more than 100 years since the first attempt to produce a cholera vaccine was made, an effective cholera vaccine has yet to be developed. In this study, the level of protection produced by a potential DNA vaccine (PVax/ctxB) was tested against the ctxB toxin of Vibrio cholerae on...

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Main Author: Moh’d Hamadneh, Lama Abdel Qader
Format: Thesis
Language:English
Published: 2003
Online Access:http://ethesis.upm.edu.my/id/eprint/2761/1/FPSK_%28M%29_2003_2_F.pdf
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id oai:ethesis.upm.edu.my:2761
record_format eprints
spelling oai:ethesis.upm.edu.my:2761 http://ethesis.upm.edu.my/id/eprint/2761/ Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae Moh’d Hamadneh, Lama Abdel Qader Although it has been more than 100 years since the first attempt to produce a cholera vaccine was made, an effective cholera vaccine has yet to be developed. In this study, the level of protection produced by a potential DNA vaccine (PVax/ctxB) was tested against the ctxB toxin of Vibrio cholerae on Balb/c mice. First, the intramuscular vaccination method was validated using pCMV plasmid that encodes HbsAg, which was detected 5 days after the injection into the tibial muscle. Next, 4 groups of mice were intramuscularly injected with either the pVax/ctxB vaccine construct or pVaxl as the negative control. The first and second groups received 2 injections spaced 3 weeks apart, while the other two groups were given 3 injections spaced 3 weeks apart. This was then followed by challenging the mice with 105 or 107 cfu/ml/mouse from clinical isolates of V. cholerae after 3 weeks of the last injection. Antibody levels for both IgG and serum IgA were monitored using ELISA, and showed high production of IgG after the first booster injection with no significant change of IgA levels. 2003-06 Thesis NonPeerReviewed application/pdf en http://ethesis.upm.edu.my/id/eprint/2761/1/FPSK_%28M%29_2003_2_F.pdf Moh’d Hamadneh, Lama Abdel Qader (2003) Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae. Masters thesis, Universiti Putra Malaysia. (FPSK (M) 2003 2).
institution UPM eTHESES
collection UPM eTHESES
language English
description Although it has been more than 100 years since the first attempt to produce a cholera vaccine was made, an effective cholera vaccine has yet to be developed. In this study, the level of protection produced by a potential DNA vaccine (PVax/ctxB) was tested against the ctxB toxin of Vibrio cholerae on Balb/c mice. First, the intramuscular vaccination method was validated using pCMV plasmid that encodes HbsAg, which was detected 5 days after the injection into the tibial muscle. Next, 4 groups of mice were intramuscularly injected with either the pVax/ctxB vaccine construct or pVaxl as the negative control. The first and second groups received 2 injections spaced 3 weeks apart, while the other two groups were given 3 injections spaced 3 weeks apart. This was then followed by challenging the mice with 105 or 107 cfu/ml/mouse from clinical isolates of V. cholerae after 3 weeks of the last injection. Antibody levels for both IgG and serum IgA were monitored using ELISA, and showed high production of IgG after the first booster injection with no significant change of IgA levels.
format Thesis
author Moh’d Hamadneh, Lama Abdel Qader
spellingShingle Moh’d Hamadneh, Lama Abdel Qader
Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
author_facet Moh’d Hamadneh, Lama Abdel Qader
author_sort Moh’d Hamadneh, Lama Abdel Qader
title Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
title_short Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
title_full Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
title_fullStr Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
title_full_unstemmed Evaluating and Testing of a Potential DNA Vaccine Against Vibrio Cholerae
title_sort evaluating and testing of a potential dna vaccine against vibrio cholerae
publishDate 2003
url http://ethesis.upm.edu.my/id/eprint/2761/1/FPSK_%28M%29_2003_2_F.pdf
_version_ 1819310293867560960
score 13.4562235

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