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Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate

The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fer...

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Main Author: Ang, Dek Chang
Format: Thesis
Language:English
Published: 2001
Online Access:http://ethesis.upm.edu.my/id/eprint/3491/1/FSMB_2001_39_F.pdf
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spelling oai:ethesis.upm.edu.my:3491 http://ethesis.upm.edu.my/id/eprint/3491/ Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate Ang, Dek Chang The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45% and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol production using various types of starch, initial starch concentration was fixed at 20 gL-1 Among different types of starch investigated, com starch was the most susceptible to the glucoamylase, followed by sago, cassava, potato and rice starch. 2001-06 Thesis NonPeerReviewed application/pdf en http://ethesis.upm.edu.my/id/eprint/3491/1/FSMB_2001_39_F.pdf Ang, Dek Chang (2001) Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate. Masters thesis, Universiti Putra Malaysia. (FSMB 2001 39).
institution UPM eTHESES
collection UPM eTHESES
language English
description The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45% and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol production using various types of starch, initial starch concentration was fixed at 20 gL-1 Among different types of starch investigated, com starch was the most susceptible to the glucoamylase, followed by sago, cassava, potato and rice starch.
format Thesis
author Ang, Dek Chang
spellingShingle Ang, Dek Chang
Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
author_facet Ang, Dek Chang
author_sort Ang, Dek Chang
title Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_short Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_full Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_fullStr Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_full_unstemmed Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_sort production of ethanol by genetically modified saccharomyces cerevisiae using sago starch as substrate
publishDate 2001
url http://ethesis.upm.edu.my/id/eprint/3491/1/FSMB_2001_39_F.pdf
_version_ 1819310376190214144
score 13.4562235

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