صورة الغلاف

Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique

The present study evaluates the effects of various loading solutions, concentrations of glycerol and vitrification solutions and their time of exposure on the vitrification of rambutan embryos in liquid nitrogen. In the initial study to evaluate the effects of loading solutions on survival, excised...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: C. Ginibun, Florence
التنسيق: أطروحة
اللغة:English
منشور في: 2001
الوصول للمادة أونلاين:http://ethesis.upm.edu.my/id/eprint/3699/1/FP_2001_18_F.pdf
الوسوم: إضافة وسم
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id oai:ethesis.upm.edu.my:3699
record_format eprints
spelling oai:ethesis.upm.edu.my:3699 http://ethesis.upm.edu.my/id/eprint/3699/ Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique C. Ginibun, Florence The present study evaluates the effects of various loading solutions, concentrations of glycerol and vitrification solutions and their time of exposure on the vitrification of rambutan embryos in liquid nitrogen. In the initial study to evaluate the effects of loading solutions on survival, excised embryos were exposed to four loading solutions. The two most promising loading solutions were LB (1.5 M glycerol + 0.4 M sucrose + 5% DMSO), which gave 44.0 % viability and 32.4 % survival and LA (2.0 M glycerol + 0.4 M sucrose) which gave 39.3 % viability and 28.1 % survival after freezing. The effects of different concentrations of glycerol (0 - 2.0 M) in the most promising loading solutions were evaluated further. For loading solution LB, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LS, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LA, 0 M glycerol or the use of only 0.4 M sucrose gave the highest viability of 76.0 % and survival of 59.0 %. Hence, loading solution with only 0.4 M sucrose (LA without glycerol) was established in this study as the most effective loading solution for rambutan embryos. The effects of exposure time (0-16 hours) to the best loading solution on survival of rambutan embryos were further investigated. It was found that 8 hours duration gave the highest viability (47.7 %) and survival (32.8 %). Having confirmed the best loading treatment, the study further evaluates the effects of six vitrification solutions on survival of rambutan embryos in liquid nitrogen. The results show that after freezing, L Solution gave the highest viability (46.0 %) and survival (24.0 %). L Solution was therefore selected as the most effective vitrification solution. In optimizing the time of exposure, excised rambutan embryos were exposed to L Solution for 0 to 90 minutes before LN exposure. The highest viability (55.6 %) and survival (40.3 %) after vitirification were achieved at 60 minutes exposure. Longer exposure to L Solution for up to 90 minutes reduced survival to 16.0 %. 2001 Thesis NonPeerReviewed application/pdf en http://ethesis.upm.edu.my/id/eprint/3699/1/FP_2001_18_F.pdf C. Ginibun, Florence (2001) Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique. Masters thesis, Universiti Putra Malaysia. (FP 2001 18).
institution UPM eTHESES
collection UPM eTHESES
language English
description The present study evaluates the effects of various loading solutions, concentrations of glycerol and vitrification solutions and their time of exposure on the vitrification of rambutan embryos in liquid nitrogen. In the initial study to evaluate the effects of loading solutions on survival, excised embryos were exposed to four loading solutions. The two most promising loading solutions were LB (1.5 M glycerol + 0.4 M sucrose + 5% DMSO), which gave 44.0 % viability and 32.4 % survival and LA (2.0 M glycerol + 0.4 M sucrose) which gave 39.3 % viability and 28.1 % survival after freezing. The effects of different concentrations of glycerol (0 - 2.0 M) in the most promising loading solutions were evaluated further. For loading solution LB, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LS, 1.5 M glycerol gave highest survival of 22.7 %. For loading solution LA, 0 M glycerol or the use of only 0.4 M sucrose gave the highest viability of 76.0 % and survival of 59.0 %. Hence, loading solution with only 0.4 M sucrose (LA without glycerol) was established in this study as the most effective loading solution for rambutan embryos. The effects of exposure time (0-16 hours) to the best loading solution on survival of rambutan embryos were further investigated. It was found that 8 hours duration gave the highest viability (47.7 %) and survival (32.8 %). Having confirmed the best loading treatment, the study further evaluates the effects of six vitrification solutions on survival of rambutan embryos in liquid nitrogen. The results show that after freezing, L Solution gave the highest viability (46.0 %) and survival (24.0 %). L Solution was therefore selected as the most effective vitrification solution. In optimizing the time of exposure, excised rambutan embryos were exposed to L Solution for 0 to 90 minutes before LN exposure. The highest viability (55.6 %) and survival (40.3 %) after vitirification were achieved at 60 minutes exposure. Longer exposure to L Solution for up to 90 minutes reduced survival to 16.0 %.
format Thesis
author C. Ginibun, Florence
spellingShingle C. Ginibun, Florence
Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
author_facet C. Ginibun, Florence
author_sort C. Ginibun, Florence
title Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
title_short Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
title_full Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
title_fullStr Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
title_full_unstemmed Cryopreservation of Excised Embryos of Rambutan (Nephelium lappaceum L.) Using Vitrification Technique
title_sort cryopreservation of excised embryos of rambutan (nephelium lappaceum l.) using vitrification technique
publishDate 2001
url http://ethesis.upm.edu.my/id/eprint/3699/1/FP_2001_18_F.pdf
_version_ 1819310399537807360
score 13.4562235

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