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Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)

Species of the entomopathogenic fungus Metarhizium are some of the most promising biocontrol agents against Lepidopteran pests. Laboratory bioassays were conducted to study the virulence of five isolates of Metarhizium sp. against larvae of the polyphagous pest, Spilarctia obliqua (Lepidoptera: Arct...

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Main Authors: Rajan, P. D., Sasidharan, T. O., Balachander, M., Sapna Bai, N., Remadevi, O. K.
Format: Journal Contribution
Language:English
Published: 2015
Subjects:
Metarhizium
Spilarctia obliqua
Biocontrol
Hosts
Lepidoptera
Coleoptera
Isoptera
Entomopathogenic fungi
Pathogenicity
Larval stages
Virulence
Chitin
Insecticides
Toxicity
Bioassays
Online Access:http://agris.upm.edu.my:8080/dspace/handle/0/10567
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spelling oai:http:--agris.upm.edu.my:0-10567Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)Rajan, P. D.Sasidharan, T. O.Balachander, M.Sapna Bai, N.Remadevi, O. K.MetarhiziumSpilarctia obliquaBiocontrolHostsLepidopteraColeopteraIsopteraEntomopathogenic fungiPathogenicityLarval stagesVirulenceChitinInsecticidesToxicityBioassaysSpecies of the entomopathogenic fungus Metarhizium are some of the most promising biocontrol agents against Lepidopteran pests. Laboratory bioassays were conducted to study the virulence of five isolates of Metarhizium sp. against larvae of the polyphagous pest, Spilarctia obliqua (Lepidoptera: Arctiidae). The Metarhizium isolates used in the study were recovered from soil and various insect hosts belonging to Lepidoptera, Coleoptera and Isoptera. Initially, a preliminary assay was carried out involving 18 Metarhizium isolates at a spore concentration 107 conidia ml-1 and five isolates, MIS 1, MIS 2, MIS 3, MIS 8 and MIS 9, which caused more than 50% mortality were chosen for the detailed bioassay. The assay was carried out with four different spore concentrations of each isolate, ranging from 104−107 conidia ml -1. The lethal concentration LC50 of these five isolates ranged from 2.1 × 105 to 38.9 × 105 conidia ml-1. The lethal time LT50 of the isolates ranged from 7.0−8.1, 6.0−7.7, 5.1−6.7, 4.6−5.4 days at concentrations 1 × 104, 1 × 105, 1 × 106, 1 × 107 conidia ml-1 respectively. The most pathogenic among the five isolates was the MIS 2 isolate with the lowest L50 of 2.11 × 105conidia ml-1 and LT50 of 4.6, 5.1, 6.0 and 7.0 days respectively at concentrations 1 × 107, 1 × 106, 1 × 105, 1 × 104conidia ml-1. Isolate MIS 3 was second in effectiveness. These two isolates showed promise for use as biocontrol agents against S. oblique.2015-12-07T04:16:47Z2015-12-07T04:16:47Z2010Journal ContributionArticleNon-RefereedJournal of Tropical Forest Science (Malaysia), 22(1), p. 74-800128-1283http://agris.upm.edu.my:8080/dspace/handle/0/10567MY2015050752enhttp://www.frim.gov.my/v1/JTFSOnline/jtfs/v22n1/74-80.pdfhttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Metarhizium
Spilarctia obliqua
Biocontrol
Hosts
Lepidoptera
Coleoptera
Isoptera
Entomopathogenic fungi
Pathogenicity
Larval stages
Virulence
Chitin
Insecticides
Toxicity
Bioassays
spellingShingle Metarhizium
Spilarctia obliqua
Biocontrol
Hosts
Lepidoptera
Coleoptera
Isoptera
Entomopathogenic fungi
Pathogenicity
Larval stages
Virulence
Chitin
Insecticides
Toxicity
Bioassays
Rajan, P. D.
Sasidharan, T. O.
Balachander, M.
Sapna Bai, N.
Remadevi, O. K.
Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
description Species of the entomopathogenic fungus Metarhizium are some of the most promising biocontrol agents against Lepidopteran pests. Laboratory bioassays were conducted to study the virulence of five isolates of Metarhizium sp. against larvae of the polyphagous pest, Spilarctia obliqua (Lepidoptera: Arctiidae). The Metarhizium isolates used in the study were recovered from soil and various insect hosts belonging to Lepidoptera, Coleoptera and Isoptera. Initially, a preliminary assay was carried out involving 18 Metarhizium isolates at a spore concentration 107 conidia ml-1 and five isolates, MIS 1, MIS 2, MIS 3, MIS 8 and MIS 9, which caused more than 50% mortality were chosen for the detailed bioassay. The assay was carried out with four different spore concentrations of each isolate, ranging from 104−107 conidia ml -1. The lethal concentration LC50 of these five isolates ranged from 2.1 × 105 to 38.9 × 105 conidia ml-1. The lethal time LT50 of the isolates ranged from 7.0−8.1, 6.0−7.7, 5.1−6.7, 4.6−5.4 days at concentrations 1 × 104, 1 × 105, 1 × 106, 1 × 107 conidia ml-1 respectively. The most pathogenic among the five isolates was the MIS 2 isolate with the lowest L50 of 2.11 × 105conidia ml-1 and LT50 of 4.6, 5.1, 6.0 and 7.0 days respectively at concentrations 1 × 107, 1 × 106, 1 × 105, 1 × 104conidia ml-1. Isolate MIS 3 was second in effectiveness. These two isolates showed promise for use as biocontrol agents against S. oblique.
format Journal Contribution
author Rajan, P. D.
Sasidharan, T. O.
Balachander, M.
Sapna Bai, N.
Remadevi, O. K.
author_facet Rajan, P. D.
Sasidharan, T. O.
Balachander, M.
Sapna Bai, N.
Remadevi, O. K.
author_sort Rajan, P. D.
title Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
title_short Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
title_full Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
title_fullStr Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
title_full_unstemmed Virulence of Metarhiziumisolates against the polyphagous defoliator pest, Spilarctia obliqua (Lepidoptera: Arctiidae)
title_sort virulence of metarhiziumisolates against the polyphagous defoliator pest, spilarctia obliqua (lepidoptera: arctiidae)
publishDate 2015
url http://agris.upm.edu.my:8080/dspace/handle/0/10567
_version_ 1819284702126669824
score 13.4562235

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