Improvement of antifungal activity of citronella oil against aspergillus flavus on rubberwood (Hevea brasiliensis) using heat curing
Optimisation of the inhibitory effect of citronella oil (10, 30 and 50 µg mL-1) with heat curing (30, 90 and 150 °C) and drying periods (1, 12 and 24 hours) against a major mould (Aspergillus flavus) found on the surface of rubberwood was investigated using response surface methodology. Specimens we...
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| Main Authors: | , , |
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| Format: | Journal Contribution |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://agris.upm.edu.my:8080/dspace/handle/0/13706 |
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| Summary: | Optimisation of the inhibitory effect of citronella oil (10, 30 and 50 µg mL-1) with heat curing (30, 90 and 150 °C) and drying
periods (1, 12 and 24 hours) against a major mould (Aspergillus flavus) found on the surface of rubberwood was investigated using response surface methodology. Specimens were incubated at 25 °C in 100% relative
humidity for 90 days and individually rated for the period it took to achieve zero mould growth on
rubberwood. Citronella oil components were analysed by gas chromatography–mass spectrometry. Citronella
oil (50 µg mL-1) with heat curing (30 and 90 °C) and drying periods (1 to 24 hours) completely inhibited
spore germination for at least 90 days. Microscopy investigation confirmed that no spore germination
was found in treated rubberwood. Citronellal (27.5%), geraniol (20.4%), citronellol (13.4%) were major
constituents of citronella oil. Heat curing may be important for transformation of components and
enhancement of antifungal activity of citronella oil. This study showed that a combination of citronella oil
and heat curing could protect rubberwood. |
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