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Evaluation of filter paper sero-assay (FIPSA) to detect three viruses

The filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked wer...

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Auteurs principaux: Norani Abdul-Samad, Tan, Lee Hoi
Format: Proceedings Paper
Langue:English
Publié: Malaysian Plant Protection Society 2018
Sujets:
Clitoria
Hibiscus
Tomato mosaic virus
Serological techniques
Plant viruses
Infection
Viruses
Antigens
Immunoassay
Densitometry
Purification
Accès en ligne:http://agris.upm.edu.my:8080/dspace/handle/0/14336
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spelling oai:http:--agris.upm.edu.my:0-14336Evaluation of filter paper sero-assay (FIPSA) to detect three virusesNorani Abdul-SamadTan, Lee HoiClitoriaHibiscusTomato mosaic virusSerological techniquesPlant virusesInfectionVirusesAntigensImmunoassayDensitometryPurificationThe filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips, the purified viruses and sap extracts of CYVV, HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10,000 dilution, 15.0 ng/ml and 1:4,800 dilution, 5.0 ng/rnl and 1:8,000 dilution, respectively.Malaysian Plant Protection SocietyKuala Lumpur, Malaysia2018-05-21T02:06:08Z2018-05-21T02:06:08Z1999Proceedings PaperArticleNon-RefereedTropical Plant Protection in the Information Age : Proceedings Plant Protection in the Tropics: Kuala Lumpur, Malaysia, 15-18 Mac 1999, p. 429-430967-9942-20-1http://agris.upm.edu.my:8080/dspace/handle/0/14336MY2018050448enMalaysiahttp://www.oceandocs.org/license
institution AGRIS
collection AGRIS
language English
topic Clitoria
Hibiscus
Tomato mosaic virus
Serological techniques
Plant viruses
Infection
Viruses
Antigens
Immunoassay
Densitometry
Purification
spellingShingle Clitoria
Hibiscus
Tomato mosaic virus
Serological techniques
Plant viruses
Infection
Viruses
Antigens
Immunoassay
Densitometry
Purification
Norani Abdul-Samad
Tan, Lee Hoi
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
description The filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips, the purified viruses and sap extracts of CYVV, HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10,000 dilution, 15.0 ng/ml and 1:4,800 dilution, 5.0 ng/rnl and 1:8,000 dilution, respectively.
format Proceedings Paper
author Norani Abdul-Samad
Tan, Lee Hoi
author_facet Norani Abdul-Samad
Tan, Lee Hoi
author_sort Norani Abdul-Samad
title Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
title_short Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
title_full Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
title_fullStr Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
title_full_unstemmed Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
title_sort evaluation of filter paper sero-assay (fipsa) to detect three viruses
publisher Malaysian Plant Protection Society
publishDate 2018
url http://agris.upm.edu.my:8080/dspace/handle/0/14336
_version_ 1819285023482707968
score 13.4562235

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