Evaluation of filter paper sero-assay (FIPSA) to detect three viruses
The filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked wer...
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Malaysian Plant Protection Society
2018
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oai:http:--agris.upm.edu.my:0-14336Evaluation of filter paper sero-assay (FIPSA) to detect three virusesNorani Abdul-SamadTan, Lee HoiClitoriaHibiscusTomato mosaic virusSerological techniquesPlant virusesInfectionVirusesAntigensImmunoassayDensitometryPurificationThe filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips, the purified viruses and sap extracts of CYVV, HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10,000 dilution, 15.0 ng/ml and 1:4,800 dilution, 5.0 ng/rnl and 1:8,000 dilution, respectively.Malaysian Plant Protection SocietyKuala Lumpur, Malaysia2018-05-21T02:06:08Z2018-05-21T02:06:08Z1999Proceedings PaperArticleNon-RefereedTropical Plant Protection in the Information Age : Proceedings Plant Protection in the Tropics: Kuala Lumpur, Malaysia, 15-18 Mac 1999, p. 429-430967-9942-20-1http://agris.upm.edu.my:8080/dspace/handle/0/14336MY2018050448enMalaysiahttp://www.oceandocs.org/license |
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| language |
English |
| topic |
Clitoria Hibiscus Tomato mosaic virus Serological techniques Plant viruses Infection Viruses Antigens Immunoassay Densitometry Purification |
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Clitoria Hibiscus Tomato mosaic virus Serological techniques Plant viruses Infection Viruses Antigens Immunoassay Densitometry Purification Norani Abdul-Samad Tan, Lee Hoi Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| description |
The filter paper sero-assay (FiPSA) was examined, tested and compared with three different plant viruses; Clitoria yellow vein virus (CYW), Hibiscus chlorotic ringspot virus (HCRY) and tomato mosaic virus (ToMY) in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips, the purified viruses and sap extracts of CYVV, HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10,000 dilution, 15.0 ng/ml and 1:4,800 dilution, 5.0 ng/rnl and 1:8,000 dilution, respectively. |
| format |
Proceedings Paper |
| author |
Norani Abdul-Samad Tan, Lee Hoi |
| author_facet |
Norani Abdul-Samad Tan, Lee Hoi |
| author_sort |
Norani Abdul-Samad |
| title |
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| title_short |
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| title_full |
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| title_fullStr |
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| title_full_unstemmed |
Evaluation of filter paper sero-assay (FIPSA) to detect three viruses |
| title_sort |
evaluation of filter paper sero-assay (fipsa) to detect three viruses |
| publisher |
Malaysian Plant Protection Society |
| publishDate |
2018 |
| url |
http://agris.upm.edu.my:8080/dspace/handle/0/14336 |
| _version_ |
1819285023482707968 |
| score |
13.4562235 |