A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes
This study describes the development of a rapid and sensitive Loop-mediated isothermal amplification assay for detection of swine DNA in adulterated meat and meat products. The need to protect consumer’s right to eat foods of their choices, has made it imperative for researchers to develop efficient...
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oai:http:--agris.upm.edu.my:0-15528A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genesAliyu, S.Igwenagu, E.Mu’azu, A.Rochman NaimAbdullahi, U. F.Wan Rohani Wan TaibPorkDNAPigsAdulterationCertificationAnalytical methodsElectrophoresisFoodFood productsGenesLysineMeat productsPolymerase chain reactionScreening testsTechniquesSwineThis study describes the development of a rapid and sensitive Loop-mediated isothermal amplification assay for detection of swine DNA in adulterated meat and meat products. The need to protect consumer’s right to eat foods of their choices, has made it imperative for researchers to develop efficient means of screening and certification of food products. Six sets of LAMP primers designed based on porcine tRNA lysine gene and ATPase subunit 8 genes were used for the assay. Amplification was carried out under constant temperature (630C), using a simple laboratory water bath. Average time spent in amplification and detection of results was 25 min. All results were visually detected and confirmed by electrophoresis. Detection limit of the assay was 0.03 femtogram (fg) much high than the PCR assay, and detection probability of the assay was 100%. Detection of 0.5% of pork spiked with 99.5% of cattle beef is indicative of the sensitivity and robustness of the assay. This could serve as a prototype for development of a sensitive and inexpensive Swine DNA LAMP detection kit.2019-03-22T07:56:50Z2019-03-22T07:56:50Z2017Journal ContributionArticleNon-RefereedInternational Food Research Journal (Malaysia), 24 (4), p. 1357-13612231-7546http://agris.upm.edu.my:8080/dspace/handle/0/15528MY2019050210enhttp://www.ifrj.upm.edu.my/24%20(04)%202017/(1).pdfMalaysiahttp://www.oceandocs.org/license |
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Pork DNA Pigs Adulteration Certification Analytical methods Electrophoresis Food Food products Genes Lysine Meat products Polymerase chain reaction Screening tests Techniques Swine |
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Pork DNA Pigs Adulteration Certification Analytical methods Electrophoresis Food Food products Genes Lysine Meat products Polymerase chain reaction Screening tests Techniques Swine Aliyu, S. Igwenagu, E. Mu’azu, A. Rochman Naim Abdullahi, U. F. Wan Rohani Wan Taib A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| description |
This study describes the development of a rapid and sensitive Loop-mediated isothermal amplification assay for detection of swine DNA in adulterated meat and meat products. The need to protect consumer’s right to eat foods of their choices, has made it imperative for researchers to develop efficient means of screening and certification of food products. Six sets of LAMP primers designed based on porcine tRNA lysine gene and ATPase subunit 8 genes were used for the assay. Amplification was carried out under constant temperature (630C), using a simple laboratory water bath. Average time spent in amplification and detection of results was
25 min. All results were visually detected and confirmed by electrophoresis. Detection limit of the assay was 0.03 femtogram (fg) much high than the PCR assay, and detection probability of
the assay was 100%. Detection of 0.5% of pork spiked with 99.5% of cattle beef is indicative of the sensitivity and robustness of the assay. This could serve as a prototype for development of a sensitive and inexpensive Swine DNA LAMP detection kit. |
| format |
Journal Contribution |
| author |
Aliyu, S. Igwenagu, E. Mu’azu, A. Rochman Naim Abdullahi, U. F. Wan Rohani Wan Taib |
| author_facet |
Aliyu, S. Igwenagu, E. Mu’azu, A. Rochman Naim Abdullahi, U. F. Wan Rohani Wan Taib |
| author_sort |
Aliyu, S. |
| title |
A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| title_short |
A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| title_full |
A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| title_fullStr |
A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| title_full_unstemmed |
A rapid and sensitive Loop-mediated isothermal amplification assay for detection of pork DNA based on porcine tRNA lys and ATPase 8 genes |
| title_sort |
rapid and sensitive loop-mediated isothermal amplification assay for detection of pork dna based on porcine trna lys and atpase 8 genes |
| publishDate |
2019 |
| url |
http://agris.upm.edu.my:8080/dspace/handle/0/15528 |
| _version_ |
1819285210179567616 |
| score |
13.4562235 |