Staff View: Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus

Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus

Nitric oxide (NO) signaling is involved in many physiological processes in vertebrates and invertebrates. In mammals, NO plays as an endothelium-derived relaxing factor in the form of a simple unstable gas, as a neurotransmitter in the central and peripheral nervous systems and as an immune effector...

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Main Authors:	Inada, M., Nguyen, T. H. Linh, Sakai, M., Itami, T., El Asely, A. M., Sudhakaran, R., Mekata, T., Yui, T., Yoshida, T., Kono, T., Okugawa, S.
Format:	Journal Contribution
Language:	English
Published:	2019
Subjects:	Prawns and shrimps Nitric oxide Gene expression Identification Neurotransmitters Nervous system Macrophages Crustaceans Immunity DNA sequence Crabs RNA Vibrio Injection Acclimatization Extraction Cloning PCR Orthoptera Gametogenesis
Online Access:	http://agris.upm.edu.my:8080/dspace/handle/0/16206
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spelling	oai:http:--agris.upm.edu.my:0-16206Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicusInada, M.Nguyen, T. H. LinhSakai, M.Itami, T.El Asely, A. M.Sudhakaran, R.Mekata, T.Yui, T.Yoshida, T.Kono, T.Okugawa, S.Prawns and shrimpsNitric oxideGene expressionIdentificationNeurotransmittersNervous systemMacrophagesCrustaceansImmunityDNA sequenceCrabsRNAVibrioInjectionAcclimatizationExtractionCloningPCROrthopteraGametogenesisNitric oxide (NO) signaling is involved in many physiological processes in vertebrates and invertebrates. In mammals, NO plays as an endothelium-derived relaxing factor in the form of a simple unstable gas, as a neurotransmitter in the central and peripheral nervous systems and as an immune effector mediated by macrophages. In crustaceans, nitric oxide synthase (NOS) plays a significant role in innate immunity and in the regulation of the nervous system. We describe the full-length cDNA sequence (4,616 bp) of the kuruma shrimp M. japonicus NOS (Mj NOS). The open reading frame of Mj NOS encoded a protein of 1,187 amino acids with an estimated mass of 134 kDa, and had an 82.3% sequence homology with the NOS gene of the land crab Gecarcinus lateralis. In the brain, gill, intestine, thoracic ganglion and testis of the kuruma shrimp Mj NOS, mRNA was constitutively expressed. When Vibrio penaeicida was injected into the kuruma shrimp, Mj NOS was expressed in the brain, gill, heart, lymphoid organ, intestine and thoracic ganglion. In the gill, Mj NOS expression reached its peak at 12 h and decreased to its normal level 24 h after V. penaeicida injection.2019-09-10T09:16:52Z2019-09-10T09:16:52Z2010Journal ContributionArticleNon-RefereedThe Journal of the Asian Fisheries Society (Malaysia), 23 (4), p. 525-5430116-6514http://agris.upm.edu.my:8080/dspace/handle/0/16206MY2019050888enhttp://www.asianfisheriessociety.org/publication/downloadfile.php?id=124&file=Y0dSbUx6QXdNRE15TURJd01ERXpOVFUzT1RRM05qWXVjR1Jt&dldname=Identification%20and%20expression%20analysis%20of%20nitric%20oxide%20synthase%20gene,%20Mj%20NOS,%20in%20kuruma%20shrimp%20Marsupenaeus%20japonicus.pdfJapanhttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Prawns and shrimps Nitric oxide Gene expression Identification Neurotransmitters Nervous system Macrophages Crustaceans Immunity DNA sequence Crabs RNA Vibrio Injection Acclimatization Extraction Cloning PCR Orthoptera Gametogenesis
spellingShingle	Prawns and shrimps Nitric oxide Gene expression Identification Neurotransmitters Nervous system Macrophages Crustaceans Immunity DNA sequence Crabs RNA Vibrio Injection Acclimatization Extraction Cloning PCR Orthoptera Gametogenesis Inada, M. Nguyen, T. H. Linh Sakai, M. Itami, T. El Asely, A. M. Sudhakaran, R. Mekata, T. Yui, T. Yoshida, T. Kono, T. Okugawa, S. Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
description	Nitric oxide (NO) signaling is involved in many physiological processes in vertebrates and invertebrates. In mammals, NO plays as an endothelium-derived relaxing factor in the form of a simple unstable gas, as a neurotransmitter in the central and peripheral nervous systems and as an immune effector mediated by macrophages. In crustaceans, nitric oxide synthase (NOS) plays a significant role in innate immunity and in the regulation of the nervous system. We describe the full-length cDNA sequence (4,616 bp) of the kuruma shrimp M. japonicus NOS (Mj NOS). The open reading frame of Mj NOS encoded a protein of 1,187 amino acids with an estimated mass of 134 kDa, and had an 82.3% sequence homology with the NOS gene of the land crab Gecarcinus lateralis. In the brain, gill, intestine, thoracic ganglion and testis of the kuruma shrimp Mj NOS, mRNA was constitutively expressed. When Vibrio penaeicida was injected into the kuruma shrimp, Mj NOS was expressed in the brain, gill, heart, lymphoid organ, intestine and thoracic ganglion. In the gill, Mj NOS expression reached its peak at 12 h and decreased to its normal level 24 h after V. penaeicida injection.
format	Journal Contribution
author	Inada, M. Nguyen, T. H. Linh Sakai, M. Itami, T. El Asely, A. M. Sudhakaran, R. Mekata, T. Yui, T. Yoshida, T. Kono, T. Okugawa, S.
author_facet	Inada, M. Nguyen, T. H. Linh Sakai, M. Itami, T. El Asely, A. M. Sudhakaran, R. Mekata, T. Yui, T. Yoshida, T. Kono, T. Okugawa, S.
author_sort	Inada, M.
title	Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
title_short	Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
title_full	Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
title_fullStr	Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
title_full_unstemmed	Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus
title_sort	identification and expression analysis of nitric oxide synthase gene, mj nos, in kuruma shrimp marsupenaeus japonicus
publishDate	2019
url	http://agris.upm.edu.my:8080/dspace/handle/0/16206
_version_	1782772173758267392
score	12.935284

Identification and expression analysis of nitric oxide synthase gene, Mj NOS, in kuruma shrimp Marsupenaeus japonicus

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