Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus
The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify ma...
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oai:http:--agris.upm.edu.my:0-16509Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genusNordiana Hanim Mohd NorSingh, RajinderSmith, S. W.Schwarzacher, TrudeNoorhariza Mohd ZakiMadon, M.Muhammad Azwan ZulkifliHeslop-Harrison, J. S.Elaeis guineensisChromosomesGenetic markersHybridizationIdentificationExtractsPurificationFluorescenceRibosomal rnaBioinformaticsPolymerase chain reactionHybridsDNA sequenceNucleotide sequenceClonesGenomesOil palmsThe development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore, the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.2019-12-05T03:21:19Z2019-12-05T03:21:19Z2017Journal ContributionArticleNon-RefereedJournal of Oil Palm Research, 29 (4), p. 594-5991511-2780http://agris.upm.edu.my:8080/dspace/handle/0/16509MY2019051190enhttp://jopr.mpob.gov.my/short-communication-towards-development-of-elaeis-guineensis-chromosome-arm-specific-markers-and-their-utility-across-the-elaeis-genus/?v=truehttps://doi.org/10.21894/jopr.2017.0001Malaysiahttp://www.oceandocs.org/license |
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| language |
English |
| topic |
Elaeis guineensis Chromosomes Genetic markers Hybridization Identification Extracts Purification Fluorescence Ribosomal rna Bioinformatics Polymerase chain reaction Hybrids DNA sequence Nucleotide sequence Clones Genomes Oil palms |
| spellingShingle |
Elaeis guineensis Chromosomes Genetic markers Hybridization Identification Extracts Purification Fluorescence Ribosomal rna Bioinformatics Polymerase chain reaction Hybrids DNA sequence Nucleotide sequence Clones Genomes Oil palms Nordiana Hanim Mohd Nor Singh, Rajinder Smith, S. W. Schwarzacher, Trude Noorhariza Mohd Zaki Madon, M. Muhammad Azwan Zulkifli Heslop-Harrison, J. S. Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| description |
The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic, sequence and chromosomal maps, valuable for comparative studies, hybridisation and breeding. Here, we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore, the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids. |
| format |
Journal Contribution |
| author |
Nordiana Hanim Mohd Nor Singh, Rajinder Smith, S. W. Schwarzacher, Trude Noorhariza Mohd Zaki Madon, M. Muhammad Azwan Zulkifli Heslop-Harrison, J. S. |
| author_facet |
Nordiana Hanim Mohd Nor Singh, Rajinder Smith, S. W. Schwarzacher, Trude Noorhariza Mohd Zaki Madon, M. Muhammad Azwan Zulkifli Heslop-Harrison, J. S. |
| author_sort |
Nordiana Hanim Mohd Nor |
| title |
Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| title_short |
Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| title_full |
Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| title_fullStr |
Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| title_full_unstemmed |
Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus |
| title_sort |
short communication: towards development of elaeis guineensis chromosome-arm specific markers and their utility across the elaeis genus |
| publishDate |
2019 |
| url |
http://agris.upm.edu.my:8080/dspace/handle/0/16509 |
| _version_ |
1782772293927174144 |
| score |
12.935284 |