Registro fonte: In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia

In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia

Mud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently, one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising...

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Principais autores:	Shumpei Ieahata, Nurul Aqilah Iberahim, Sharifah Rahmah Syed Muhammad, Alia Syafiqah Aznan, Khairulbariyyah Zakaria, Nadirah Musa, Muhd Danish Daniel Abdullah, Wan Nurhafizah Wan Ibrahim, Najiah Musa, Sharifah Noor Emilia Syed Jamil Fadaak, Lee, Kok Leong, Nur Azna Saari, Yeong, Yik Sung, Laith Abdul Razzak, Mohamed Tajuddin Abdul Manaf, Sandra Catherine Zainathan, Nor Hazwani Hamzah, Mazlan Abd Ghaffar
Formato:	Journal Contribution
Idioma:	English
Publicado em:	2020
Assuntos:	Scylla olivacea Probiotics Antibacterial agents Aquatic animals Aquatic organisms In vitro Evaluation Sampling Dry season Centrifugation Homogenization Laboratory animals Antibiotics Bile salts Vibrio Vibrio parahaemolyticus Vibrio cholerae Isolation Identification Bacillus licheniformis Antibiotic resistance Intestines Pathogens Tolerance Trypsin Pepsin Wetlands
Acesso em linha:	http://agris.upm.edu.my:8080/dspace/handle/0/17419
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spelling	oai:http:--agris.upm.edu.my:0-17419In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, MalaysiaShumpei IeahataNurul Aqilah IberahimSharifah Rahmah Syed MuhammadAlia Syafiqah AznanKhairulbariyyah ZakariaNadirah MusaMuhd Danish Daniel AbdullahWan Nurhafizah Wan IbrahimNajiah MusaSharifah Noor Emilia Syed Jamil FadaakLee, Kok LeongNur Azna SaariYeong, Yik SungLaith Abdul RazzakMohamed Tajuddin Abdul ManafSandra Catherine ZainathanNor Hazwani HamzahMazlan Abd GhaffarScylla olivaceaProbioticsAntibacterial agentsAquatic animalsAquatic organismsIn vitroEvaluationSamplingDry seasonCentrifugationHomogenizationLaboratory animalsAntibioticsBile saltsVibrioVibrio parahaemolyticusVibrio choleraeIsolationIdentificationBacillus licheniformisAntibiotic resistanceIntestinesPathogensToleranceTrypsinPepsinWetlandsMud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently, one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising approach in aquaculture to prevent infections by pathogenic bacteria, and has been shown to help reduce mortality after infection. The goals of this study were to isolate, identify and evaluate potential probiotic bacteria from the gut of wild orange mud crab from Setiu Wetlands, Terengganu. Isolate was identified using BBLTM Crystal Gram Positive (GP) Identification System. Probiotic properties were evaluated including antibiotic susceptibility, tolerances to pH, bile salt, trypsin and pepsin, autoaggregation and coaggregation with Vibrio pathogens. Isolate UMTK003 was identified as Bacillus licheniformis. The isolate was able to withstand pH 4.5 to 9.5. Bile salt, pepsin and trypsin tolerances were 0.1-0.4%, 0.1-0.7% and 0.4-1%, respectively. Antibiotic susceptibility profiling showed multiple antibiotic resistance (MAR) index of 0.15. Autoaggregation was 79.83±0.0015%. Coaggregation varied from 57.71±0.0050% (Vibrioharveyi),52.41±0.0011% (Vibrio metschnikovii), 50.07±0.0011% (Vibrio cholera) to 43.25±0.0010% (Vibrio parahaemolyticus). These results suggested probiotic potential of B. licheniformis strain UMTK003 for mud crab aquaculture in Malaysia.2020-06-30T07:54:26Z2020-06-30T07:54:26Z2017Journal ContributionArticleNon-RefereedJournal of Sustainability Science and Management Special Issue (3), p. 62-741823-8556http://agris.upm.edu.my:8080/dspace/handle/0/17419MY2020050725enhttp://jssm.umt.edu.my/wp-content/uploads/sites/51/2017/12/Chapter-8-1.pdfMalaysiahttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Scylla olivacea Probiotics Antibacterial agents Aquatic animals Aquatic organisms In vitro Evaluation Sampling Dry season Centrifugation Homogenization Laboratory animals Antibiotics Bile salts Vibrio Vibrio parahaemolyticus Vibrio cholerae Isolation Identification Bacillus licheniformis Antibiotic resistance Intestines Pathogens Tolerance Trypsin Pepsin Wetlands
spellingShingle	Scylla olivacea Probiotics Antibacterial agents Aquatic animals Aquatic organisms In vitro Evaluation Sampling Dry season Centrifugation Homogenization Laboratory animals Antibiotics Bile salts Vibrio Vibrio parahaemolyticus Vibrio cholerae Isolation Identification Bacillus licheniformis Antibiotic resistance Intestines Pathogens Tolerance Trypsin Pepsin Wetlands Shumpei Ieahata Nurul Aqilah Iberahim Sharifah Rahmah Syed Muhammad Alia Syafiqah Aznan Khairulbariyyah Zakaria Nadirah Musa Muhd Danish Daniel Abdullah Wan Nurhafizah Wan Ibrahim Najiah Musa Sharifah Noor Emilia Syed Jamil Fadaak Lee, Kok Leong Nur Azna Saari Yeong, Yik Sung Laith Abdul Razzak Mohamed Tajuddin Abdul Manaf Sandra Catherine Zainathan Nor Hazwani Hamzah Mazlan Abd Ghaffar In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
description	Mud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently, one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising approach in aquaculture to prevent infections by pathogenic bacteria, and has been shown to help reduce mortality after infection. The goals of this study were to isolate, identify and evaluate potential probiotic bacteria from the gut of wild orange mud crab from Setiu Wetlands, Terengganu. Isolate was identified using BBLTM Crystal Gram Positive (GP) Identification System. Probiotic properties were evaluated including antibiotic susceptibility, tolerances to pH, bile salt, trypsin and pepsin, autoaggregation and coaggregation with Vibrio pathogens. Isolate UMTK003 was identified as Bacillus licheniformis. The isolate was able to withstand pH 4.5 to 9.5. Bile salt, pepsin and trypsin tolerances were 0.1-0.4%, 0.1-0.7% and 0.4-1%, respectively. Antibiotic susceptibility profiling showed multiple antibiotic resistance (MAR) index of 0.15. Autoaggregation was 79.83±0.0015%. Coaggregation varied from 57.71±0.0050% (Vibrioharveyi),52.41±0.0011% (Vibrio metschnikovii), 50.07±0.0011% (Vibrio cholera) to 43.25±0.0010% (Vibrio parahaemolyticus). These results suggested probiotic potential of B. licheniformis strain UMTK003 for mud crab aquaculture in Malaysia.
format	Journal Contribution
author	Shumpei Ieahata Nurul Aqilah Iberahim Sharifah Rahmah Syed Muhammad Alia Syafiqah Aznan Khairulbariyyah Zakaria Nadirah Musa Muhd Danish Daniel Abdullah Wan Nurhafizah Wan Ibrahim Najiah Musa Sharifah Noor Emilia Syed Jamil Fadaak Lee, Kok Leong Nur Azna Saari Yeong, Yik Sung Laith Abdul Razzak Mohamed Tajuddin Abdul Manaf Sandra Catherine Zainathan Nor Hazwani Hamzah Mazlan Abd Ghaffar
author_facet	Shumpei Ieahata Nurul Aqilah Iberahim Sharifah Rahmah Syed Muhammad Alia Syafiqah Aznan Khairulbariyyah Zakaria Nadirah Musa Muhd Danish Daniel Abdullah Wan Nurhafizah Wan Ibrahim Najiah Musa Sharifah Noor Emilia Syed Jamil Fadaak Lee, Kok Leong Nur Azna Saari Yeong, Yik Sung Laith Abdul Razzak Mohamed Tajuddin Abdul Manaf Sandra Catherine Zainathan Nor Hazwani Hamzah Mazlan Abd Ghaffar
author_sort	Shumpei Ieahata
title	In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
title_short	In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
title_full	In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
title_fullStr	In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
title_full_unstemmed	In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia
title_sort	in vitro evaluation of probiotic properties of bacillus licheniformis strain umtk003 from gut of wild orange mud crab, scylla olivacea from setiu wetlands, terengganu, malaysia
publishDate	2020
url	http://agris.upm.edu.my:8080/dspace/handle/0/17419
_version_	1819285285220909056
score	13.4562235

In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia

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